Rapid testing of gut permeability using oral fluorescein and confocal laser endomicroscopy in Zambian adults.
Rapid testing of gut permeability using oral fluorescein and confocal laser endomicroscopy in Zambian adults.
复制标题
使用口服荧光素和共焦激光内窥镜对赞比亚成年人进行肠道通透性快速检测。
DOI：
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复制
发表时间：
2021
影响因子：
2.2
通讯作者：
V. Kayamba
中科院分区：
文献类型：
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作者：
Reuben Mbuki;Sandra Chileya;A. Thompson;P. Kelly;V. Kayamba
背景
肠道通透性的双糖测试既耗时又复杂。我们探索了荧光素作为一种简单、廉价的替代方法的应用。
方法
我们使用放置在摄入荧光素钠的参与者指尖的共焦激光内窥镜探头来测量不同时间点的荧光。
结果
在10例腹泻患者和10例对照组中，荧光检测迅速。到6min时，所有腹泻患者都检测到荧光，但只有1名对照组检测到荧光(p=0.0004)。15分钟后，两组之间没有差异。
结论
这种简单的口服荧光素测试可能有助于研究低资源环境下的肠道通透性。
BACKGROUND
Dual sugar testing for gut permeability is time-consuming and complex. We explored the utility of fluorescein as a simple and inexpensive alternative method.
METHODS
We used a confocal laser endomicroscopy probe placed at the fingertip of participants who had ingested sodium fluorescein to measure the fluorescence at various time points.
RESULTS
In 10 patients with diarrhoea, but not in 10 controls, fluorescence was detected quickly. By 6 min fluorescence was detected in all diarrhoea patients but only 1 control (p=0.0004). After 15 min there was no difference between the groups.
CONCLUSIONS
This simple oral fluorescein test may be useful to study gut permeability in low-resource settings.
影响因子：
4.3
作者：
Klingensmith NJ;Coopersmith CM
通讯作者：
Coopersmith CM