Isolation and Identification of a Microorganism Producing Bilirubin Oxidase
Isolation and Identification of a Microorganism Producing Bilirubin Oxidase
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一株产胆红素氧化酶微生物的分离与鉴定
DOI：
10.1271/bbb1961.46.2031
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
N. Tanaka
中科院分区：
文献类型：
--
作者：
S. Murao;N. Tanaka
为了使原污水脱色并用作临床领域的分析工具，我们试图获得产生对胆红素具有反应性的酶的微生物。对一株产酶能力较强的微生物MT-1进行了形态学和生理生化特性的研究。该菌株被发现属于疣状漆斑菌（Myrothecium verrucaria）。为了生产该酶，将该菌株在含有马铃薯葡萄糖培养基的罐式发酵罐中于25°C下有氧培养。该酶在培养62小时后活性最高，其它漆斑菌属菌株也能产生该酶。
For the purposes of decoloring raw sewage and use as an analytical tool in clinical fields, we tried to obtain microorganisms producing an enzyme which is reactive to bilirubin. One strain of microorganism (MT-1) showed strong ability to produce the enzyme.The morphological and physiological characteristics of strain MT-1 were studied. This strain was found to belong to Myrothecium verrucaria.For the production of the enzyme, this strain was aerobically cultured at 25°C in a jar fermentor which contained potato-glucose medium. The highest activity was obtained after 62-hr cultivation.This enzyme was also produced by other strains belonging to Myrothecium.