Mapping bacterial surface population physiology in real-time:: Infrared spectroscopy of Proteus mirabilis swarm colonies
Mapping bacterial surface population physiology in real-time:: Infrared spectroscopy of Proteus mirabilis swarm colonies
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DOI：
10.1366/000370206777670558
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发表时间：
2006-06-01
影响因子：
3.5
通讯作者：
Sire, Olivier
中科院分区：
文献类型：
--
作者：
Keirsse, Julie;Lahaye, Elodie;Sire, Olivier
我们通过红外 (IR) 显微光谱绘制了生长中的奇异变形杆菌菌落内静止细胞和集群细胞的时空分布。使用安装在显微镜上的离散红外传感器在接种物和外围之间的不同位置进行菌落绘图，同时使用基于光纤的红外衰减全反射（ATR）传感器或“光极”在固定位置随时间连续监测。单个奇异果群体内的表型依赖于功能决定因素（产生独特的光谱信号）的识别，这些决定因素反映了与细胞分化相关的大分子组成的差异。内部群落区域在光谱上是均匀的，具有与接种物产生的图案相似的图案。由活跃的集群细胞组成的外部区域表现出在以多糖为主的多个波长下可区分的光谱。我们的实时观察结果与早期的报告一致并延伸，表明活动的集群细胞被限制在集落边缘的狭窄（约 3 毫米）环内。因此，这项研究验证了红外光极可以用于生物膜和其他细菌表面种群的实时和无创监测。
We mapped the space-time distribution of stationary and swarmer cells within a growing Proteus mirabilis colony by infrared (IR) microspectroscopy. Colony mapping was performed at different positions between the inoculum and the periphery with a discrete microscope-mounted IR sensor, while continuous monitoring at a fixed location over time used an optical fiber based IR-attenuated total reflection (ATR) sensor, or "optrode." Phenotypes within a single P mirabilis population relied on identification of functional determinants (producing unique spectral signals) that reflect differences in macromolecular composition associated with cell differentiation. Inner swarm colony domains are spectrally homogeneous, having patterns similar to those produced by the inoculum. Outer domains composed of active swarmer cells exhibit spectra distinguishable at multiple wavelengths dominated by polysaccharides. Our real-time observations agree with and extend earlier reports indicating that motile swarmer cells are restricted to a narrow (approximately 3 mm) annulus at the colony edge. This study thus validates the use of an IR optrode for real-time and noninvasive monitoring of biofilms and other bacterial surface populations.