Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology
Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology
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DOI：
10.1021/la5029993
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发表时间：
2015-04-14
期刊：
影响因子：
3.9
通讯作者：
Chattopadhyay, Krishnananda
中科院分区：
文献类型：
--
作者：
Haldar, Shubhasis;Sil, Pallabi;Chattopadhyay, Krishnananda
不同蛋白质和疾病系统中发现了具有不同形态的淀粉样物质。在这篇文章中，我们的目的是问这些形态是否是特定蛋白质所独有的，或者它们是否从一种转化为另一种。使用含有铁的血红素蛋白作为聚集的过渡金属激活剂和带负电荷的表面活性剂，部分展开的蛋白质和其聚集已被诱导。在聚集途径中，我们观察到单个蛋白质的几种形态结构的形成，这些结构直接使用原子力显微镜（AFM）可视化。已经发现这些结构随时间出现和消失，并且它们的形成可以在正常缓冲液条件下和室温下监测，而不需要任何复杂的化学或生物学方法。此外，我们还观察到蜂窝状形态的形成，这可能是一个中间体。这些基于淀粉样蛋白的纳米结构可能具有在治疗递送和其他生物医学应用中探索的潜力。
Amyloid species with various morphologies have been found for different proteins and disease systems. In this article, we aim to ask if these morphologies are unique to a particular protein or if they convert from one to another. Using a heme protein containing iron as the transition-metal activator of aggregation and a negatively charged surfactant, partial unfolding of the protein and its aggregation have been induced. In the pathway of aggregation, we have observed the formation of several morphological structures of a single protein, which were visualized directly using atomic force microscopy (AFM). These structures have been found to appear and disappear with time, and their formation could be monitored under normal buffer conditions and at room temperature without requiring any sophisticated chemical or biological methodologies. In addition, we have observed the formation of honeycomb-shaped morphology, which may serve as an intermediate. These amyloid-based nanostructures may have the potential to be explored in therapeutics delivery and other biomedical applications.