CHARACTERIZATION OF A THERMOPHILIC BACTERIOPHAGE FOR BACILLUS STEAROTHERMOPHILUS
CHARACTERIZATION OF A THERMOPHILIC BACTERIOPHAGE FOR BACILLUS STEAROTHERMOPHILUS
复制标题
DOI：
10.1128/jb.91.1.340-348.1966
复制
发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
CAMPBELL, LL
中科院分区：
文献类型：
--
作者：
SAUNDERS, GF;CAMPBELL, LL
研究了嗜热噬菌体TP-84的生物学和理化性质。TP-84在43 - 76 ℃的温度范围内对24株嗜热脂肪芽孢杆菌菌株中的3株有溶解作用. TP-84对B. TP-84具有六边形头部，直径为53 m[mu]，边长为30 m[mu];其尾部为130 m [mu]长，3 - 5 m[mu]宽。该噬菌体的S20、W为436，在CsCl（pH 8.5）中的条带密度为1.508 g/cc。TP-84的扩散系数计算为6.19 x 10-8 cm 2/sec。根据沉降和扩散数据，计算出TP-84的颗粒分子量为5000万道尔顿。噬菌体DNA具有42%鸟嘌呤+胞嘧啶的碱基组成，从浮力密度和解链温度测量推断。
The biological and physical-chemical properties of the thermophilic bacteriophage TP-84 were investigated. TP-84 was shown to be lytic for 3 of 24 strains of Bacillus stearothermophilus tested over the temperature range of 43 to 76 C. The latent period of TP-84 on B. stearothermophilus strain 10 was 22 to 24 min. TP-84 has a hexagonal head, 53 m[mu] in diameter and 30 m[mu] on a side; its tail is 130 m[mu] long and 3 to 5 m[mu] wide. The phage has an S20, W of 436, and bands at a density of 1.508 g/cc in CsCl (pH 8.5). The diffusion coefficient of TP-84 was calculated to be 6.19 x 10-8 cm2/sec. From the sedimentation and diffusion data, a particle molecular weight of 50 million daltons was calculated for TP-84. The phage DNA has a base composition of 42% guanine + cytosine, deduced from buoyant density and melting temperature measurements.