Adaptive evolution of an essential telomere protein restricts telomeric retrotransposons.
Adaptive evolution of an essential telomere protein restricts telomeric retrotransposons.
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一种必需端粒蛋白的适应性进化限制了端粒逆转录转座子。
DOI：
10.7554/elife.60987
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发表时间：
2020-12-22
期刊：
影响因子：
7.7
通讯作者：
Levine MT
中科院分区：
文献类型：
--
作者：
Saint-Leandre B;Christopher C;Levine MT
必需的、保守的细胞过程不仅依赖于必需的、严格保守的蛋白质，而且依赖于快速进化的必需蛋白质。为了探索这个鲜为人知的悖论，我们利用了快速进化的果蝇端粒结合蛋白，cav/HOAP，它保护染色体免于致命的端到端融合。我们替换了D。melanogaster HOAP与其近亲D.日本料理。地方yakuba HOAP（'HOAP [yak'）定位于D. melanogaster端粒和保护D.黑腹染色体融合。然而，HOAP[yak]未能拯救以前未表征的HOAP功能：沉默专门的端粒逆转录转座子，而不是端粒酶，维持果蝇的染色体长度。实验进化群体的全基因组测序和细胞遗传学显示，HOAP[牦牛]触发端粒反转录转座子增殖，导致异常长的端粒。这种进化产生的功能分离等位基因解决了一个矛盾的观察，即快速进化的必需基因指导一个必需的，严格保守的功能：端粒逆转录转座子遏制，而不是末端保护，需要在HOAP的进化创新。
Essential, conserved cellular processes depend not only on essential, strictly conserved proteins but also on essential proteins that evolve rapidly. To probe this poorly understood paradox, we exploited the rapidly evolving Drosophila telomere-binding protein, cav/HOAP, which protects chromosomes from lethal end-to-end fusions. We replaced the D. melanogaster HOAP with a highly diverged version from its close relative, D. yakuba. The D. yakuba HOAP ('HOAP[yak]') localizes to D. melanogaster telomeres and protects D. melanogaster chromosomes from fusions. However, HOAP[yak] fails to rescue a previously uncharacterized HOAP function: silencing of the specialized telomeric retrotransposons that, instead of telomerase, maintain chromosome length in Drosophila. Whole genome sequencing and cytogenetics of experimentally evolved populations revealed that HOAP[yak] triggers telomeric retrotransposon proliferation, resulting in aberrantly long telomeres. This evolution-generated, separation-of-function allele resolves the paradoxical observation that a fast-evolving essential gene directs an essential, strictly conserved function: telomeric retrotransposon containment, not end-protection, requires evolutionary innovation at HOAP.
MTV 为果蝇的端粒生物学欢呼。
影响因子：
1.2
作者：
Cheng L;Cui M;Rong YS
通讯作者：
Rong YS