Transmembrane signalling by the N-formyl peptide receptor in stably transfected fibroblasts.
Transmembrane signalling by the N-formyl peptide receptor in stably transfected fibroblasts.
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DOI：
10.1016/0006-291x(91)91394-r
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发表时间：
1991-08
影响因子：
3.1
通讯作者：
E. Prossnitz;O. Quehenberger;C. Cochrane;R. Ye
中科院分区：
文献类型：
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作者：
E. Prossnitz;O. Quehenberger;C. Cochrane;R. Ye
我们研究了N-甲酰肽受体介导的跨膜信号在转染的小鼠成纤维细胞表达受体的要求。稳定转染的细胞显示出对N-甲酰基-Met-Leu-[3 H]Phe的特异性结合，解离常数为3 nM。细胞响应配体刺激，从细胞内储存的钙动员。钙动员是配体剂量依赖性的（EC 50 =3 nM fMet-Leu-Phe），并可被百日咳毒素处理抑制。这些结果提供了第一个证明，在小鼠成纤维细胞中的单链N-甲酰肽受体的表达足以介导配体诱导的早期跨膜信号传导事件，这似乎不需要其他嗜酸性粒细胞特异性细胞成分。
We investigated the requirement for N-formyl peptide receptor-mediated transmembrane signalling in transfected mouse fibroblasts that express the receptor. Stably transfected cells displayed specific binding for N-formyl-Met-Leu-[3H]Phe with a dissociation constant of 3 nM. The cells responded to ligand stimulation with mobilization of calcium from intracellular stores. Calcium mobilization was ligand dose-dependent (EC50=3 nM fMet-Leu-Phe) and could be inhibited by pertussis toxin treatment. These results provide the first demonstration that expression of the single-chain N-formyl peptide receptor in mouse fibroblasts is sufficient for mediating ligand-induced early transmembrane signalling events, which do not appear to require other neutrophil-specific cellular components.