Composition and Phylogenetic Analysis of Vitellogenin Coding Sequences in the Indonesian Coelacanth Latimeria menadoensis
Composition and Phylogenetic Analysis of Vitellogenin Coding Sequences in the Indonesian Coelacanth Latimeria menadoensis
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DOI：
10.1002/jez.b.22455
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发表时间：
2012-07-01
影响因子：
2.2
通讯作者：
Barucca, Marco
中科院分区：
文献类型：
--
作者：
Canapa, Adriana;Olmo, Ettore;Barucca, Marco
腔棘鱼Latimeria menadoensis是一种活化石，在探索影响陆地水生脊椎动物基因组的变化方面占据着关键的系统发育位置。本研究首次分离并分析了L. menadoensis mRNA三种不同的卵黄蛋白原转录本进行了鉴定，其推断的氨基酸序列相比，其他已知的脊椎动物。系统发育数据表明，该基因家族在腔棘鱼的进化历史的特点是不同的复制事件比那些发生在硬骨鱼类，鱼类和两栖类。三个序列的比较突出了功能位点的差异。此外，尽管与其他卵生脊椎动物相比存在保守的位点，但有些位点已经发生了变化，有些位点仅与硬骨鱼类相似，有些位点仍与四足动物相似。J. Exp. Zool.（Mol. Dev. Evol.）318B：404-416，2012. (c)2012 Wiley Periodicals，Inc.
The coelacanth Latimeria menadoensis, a living fossil, occupies a key phylogenetic position to explore the changes that have affected the genomes of the aquatic vertebrates that colonized dry land. This is the first study to isolate and analyze L. menadoensis mRNA. Three different vitellogenin transcripts were identified and their inferred amino acid sequences compared to those of other known vertebrates. The phylogenetic data suggest that the evolutionary history of this gene family in coelacanths was characterized by a different duplication event than those which occurred in teleosts, amniotes, and amphibia. Comparison of the three sequences highlighted differences in functional sites. Moreover, despite the presence of conserved sites compared with the other oviparous vertebrates, some sites were seen to have changed, others to be similar only to those of teleosts, and others still to resemble only to those of tetrapods. J. Exp. Zool. (Mol. Dev. Evol.) 318B:404-416, 2012. (c) 2012 Wiley Periodicals, Inc.