Facile and sensitive detection of protamine by enhanced room-temperature phosphorescence of Mn-doped ZnS quantum dots.
Facile and sensitive detection of protamine by enhanced room-temperature phosphorescence of Mn-doped ZnS quantum dots.
复制标题
DOI：
10.1016/j.ab.2015.03.010
复制
发表时间：
2015-06
影响因子：
2.9
通讯作者：
Zhifeng Zhang;Y. Miao;Qindi Zhang;Guiqin Yan
中科院分区：
文献类型：
--
作者：
Zhifeng Zhang;Y. Miao;Qindi Zhang;Guiqin Yan
量子点（QD）纳米杂化材料为探索材料的新特性提供了一条有效的途径，并越来越多地用作高价值的灵敏（生物）化学探针。有趣的是，3-巯基丙酸（MPA）覆盖的Mn掺杂的ZnS量子点的室温磷光（RTP）可以显着增强通过添加鱼精蛋白。基于上述发现，我们成功地设计了一种简单、灵敏、选择性好的鱼精蛋白快速检测方法。在此方法中，鱼精蛋白作为阳离子肽与MPA-capped Mn-doped ZnS量子点发生静电相互作用，形成MPA-capped Mn-doped ZnS QD/鱼精蛋白复合物，导致量子点聚集，增强RTP强度。在优化的条件下，RTP强度变化与鱼精蛋白的浓度在0.2-3.0 μg ml-1范围内呈线性关系，检测限为0.14 μg ml-1。方法用于硫酸鱼精蛋白注射液和人血清中鱼精蛋白的测定，回收率在96.5 ~ 105.6%之间，结果令人满意。
Quantum dot (QD) nanohybrids provide an effective route to explore the new properties of materials and are increasingly used as highly valuable sensitive (bio) chemical probes. Interestingly, the room-temperature phosphorescence (RTP) of 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnS QDs could be remarkably enhanced by the addition of protamine. Based on the above finding, a simple, sensitive, and selective method for rapid detection of protamine was successfully designed. With this method, protamine as a cationic peptide interacts electrostatically with MPA-capped Mn-doped ZnS QDs to form MPA-capped Mn-doped ZnS QD/protamine complexes, which leads to the aggregation of QDs and enhances the RTP intensity. Under the optimized conditions, the RTP intensity change was linearly proportional to the concentration of protamine in the range 0.2–3.0 μg ml−1, and the limit of detection was 0.14 μg ml−1. The proposed method was successfully applied to detect protamine in protamine sulfate injection and human serum samples with satisfactory results, and the recovery ranged from 96.5 to 105.6%.