Reproductive function for a C-terminus extended, male-transmitted cytochrome c oxidase subunit II protein expressed in both spermatozoa and eggs
Reproductive function for a C-terminus extended, male-transmitted cytochrome c oxidase subunit II protein expressed in both spermatozoa and eggs
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DOI：
10.1016/j.febslet.2007.10.006
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发表时间：
2007-11-13
期刊：
影响因子：
3.5
通讯作者：
Hoeh, W. R.
中科院分区：
文献类型：
--
作者：
Chakrabarti, R.;Walker, J. M.;Hoeh, W. R.
我们先前的研究记录了雄性传递的细胞色素C氧化酶亚基II蛋白(MCOX2)在双壳类动物睾丸和精子线粒体中的表达，该蛋白具有C末端延伸(MCOX2e)。在这里，我们提出的证据表明，MCOX2在睾丸中季节性表达，在受精前不久达到一个峰值，与精子密度无关。MCOX2定位于精子的内外线粒体膜，MCOX2抗体的表位在6500万年的进化过程中是保守的。我们还证明了在卵巢中存在男性传播的线粒体DNA和特定季节的MCOX2空间变异。我们假设MCOX2通过配子成熟、受精和/或胚胎发生在生殖过程中发挥作用。由Elsevier B.V.代表欧洲生化学会联合会出版。
Our previous study documented expression of a male-transmitted cytochrome c oxidase subunit II protein (MCOX2), with a C-terminus extension (MCOX2e), in unionoidean bivalve testes and sperm mitochondria. Here, we present evidence demonstrating that MCOX2 is seasonally expressed in testis, with a peak shortly before fertilization that is independent of sperm density. MCOX2 is localized to the inner and outer sperm mitochondrial membranes and the MCOX2 antibody's epitope is conserved across > 65 million years of evolution. We also demonstrate the presence of male-transmitted mtDNA and season-specific MCOX2 spatial variation in ovaries. We hypothesize that MCOX2 plays a role in reproduction through gamete maturation, fertilization and/or embryogenesis. Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies.