Parental diagnosis of satsuma mandarin (Citrus unshiu Marc.) revealed by nuclear and cytoplasmic markers.
Parental diagnosis of satsuma mandarin (Citrus unshiu Marc.) revealed by nuclear and cytoplasmic markers.
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核和细胞质标志物揭示的父母诊断Satsuma普通话（柑橘类citrus unshiu Marc。）。
DOI：
10.1270/jsbbs.16060
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发表时间：
2016-12
期刊：
影响因子：
2.4
通讯作者：
Shimada T
中科院分区：
文献类型：
--
作者：
Fujii H;Ohta S;Nonaka K;Katayose Y;Matsumoto T;Endo T;Yoshioka T;Omura M;Shimada T
温州蜜柑（Citrus unshiu Marc.）是日本主要的柑橘栽培品种。澄清其起源将被证明是有价值的柑橘分类和柑橘育种计划，但目前的信息是有限的。我们应用384个柑橘单核苷酸多态性（SNP）芯片和MARCO计算机软件进行全基因组基因分型，以调查温州蜜柑的亲子关系。从206个有效的SNPs的基因分型数据，以评估67个柑橘品种和系。MARCO基于206个SNP基因型新发现了5个亲子关系，表明'Kishuumikan'型亲核蛋白（Citrus kinokuni hort.ex Tanaka accession 'Kishuumikan'和'Nanfengmiju'）和'Kunenbo'型亲核蛋白（Citrus nobilis Lour.变种kunip Tanaka登录'Kunenbo'和'Bendiguangju'）可能是温州蜜柑的亲本。此外，酶切扩增多态性序列分析表明，“Kishuumikan”型甜菜色素叶绿体DNA中的4个区域的基因型与温州蜜柑的基因型相同。考虑到历史背景，温州蜜柑种子蛋白可能来源于“Kishuu mikan”型柑橘种子亲本（“南风蜜桔”的衍生物或同义物）和“Kunenbo”型柑橘花粉亲本（“本地蜜桔”的衍生物或同义物）之间的偶然杂交。
Satsuma mandarins (Citrus unshiu Marc.) are the predominant cultivated citrus variety in Japan. Clarification of its origin would prove valuable for citrus taxonomy and mandarin breeding programs; however, current information is limited. We applied genome-wide genotyping using a 384 citrus single nucleotide polymorphism (SNP) array and MARCO computer software to investigate the satsuma mandarin parentage. Genotyping data from 206 validated SNPs were obtained to evaluate 67 citrus varieties and lines. A total of five parent–offspring relationships were newly found by MARCO based on the 206 SNP genotypes, indicating that ‘Kishuu mikan’ type mandarins (Citrus kinokuni hort. ex Tanaka accession ‘Kishuu mikan’ and ‘Nanfengmiju’) and ‘Kunenbo’ type mandarins (Citrus nobilis Lour. var. kunip Tanaka accession ‘Kunenbo’ and ‘Bendiguangju’) are possible parents of the satsuma mandarin. Moreover, cleaved amplified polymorphic sequences analysis showed that the genotypes of four regions in chloroplast DNA of ‘Kishuu mikan’ type mandarins were identical to that of the satsuma mandarin. Considering the historical background, satsuma mandarins may therefore derive from an occasional cross between a ‘Kishuu mikan’ type mandarin seed parent (derivative or synonym of ‘Nanfengmiju’) and a ‘Kunenbo’ type mandarin pollen parent (derivative or synonym of ‘Bendiguangju’).