DETECTION OF CIRCULATING DNA BY COUNTERIMMUNOELECTROPHORESIS (CIE)
DETECTION OF CIRCULATING DNA BY COUNTERIMMUNOELECTROPHORESIS (CIE)
复制标题
DOI：
10.1002/art.1780160108
复制
发表时间：
1973-01-01
影响因子：
--
通讯作者：
DAVIS, JS
中科院分区：
文献类型：
--
作者：
DAVIS, GL;DAVIS, JS
用反向免疫电泳法可检测到血浆中低至1.5 μ g/ml的DNA和血清中低至0.2 μg/ml的DNA。然而，血清不适合这项研究，因为在凝血过程中DNA会零星地释放到血清中。在DNA检测中，对流免疫电泳的灵敏度至少是简单免疫扩散的10倍。在本研究中描述的各种疾病的血浆中发现了DNA。
Using counterimmunoelectrophoresis, DNA may be detected at concentrations as low as 1.5 μ/ml inplasmaand 0.2 μg/ml inserum. Serum, however, wasnotsuitable for this study because DNA was sporadically released into serum during the clotting process. Counterimmunoelectrophoresis is at least ten times more sensitive than simple immunodiffusion in the detection of DNA. DNA was found in plasma in a variety of illnesses which are delineated in this study.