A sex-specific, temperature-sensitive maternal effect of the daughterless mutation of Drosophila melanogaster.
A sex-specific, temperature-sensitive maternal effect of the daughterless mutation of Drosophila melanogaster.
复制标题
果蝇无女儿突变的性别特异性、温度敏感母体效应。
作者：
T. Cline
无子代（2-41.5）母系效应突变的表型各方面均表现出强烈的温度敏感性。在22度以上，da/da母亲不生女儿；然而，如果卵子发生的最后60小时和胚胎发育的前3小时发生在18度的温度下，雌性后代确实能存活下来。在这些条件下存活下来的雌虫在发育过程中表现出多种成虫角质层结构的形态异常，具有细胞死亡的特征。相比之下，它们的男性兄弟姐妹在形态上是正常的。在29度的长时间暴露后，da/da雌性不育，但继续产卵。da/da母系的有性嵌合后代即使在25度下也能存活，但这些嵌合体中雌性组织的分布和发育是异常的。这表明da+基因在卵子形成过程中具有多种功能，其中一种功能可能是雌性细胞在整个胚胎中存活所必需的。除了在卵发生过程中的作用之外，da突变在两性后代中都是一种隐性温度敏感致死突变，在胚胎发育的前半期具有TSP。
Every aspect of the phenotype of the daughterless (2-41.5) maternal effect mutation was found to be strongly temperature sensitive. Above 22 degrees, da/da mothers produced no daughters; however female progeny did survive the da maternal effect if the last 60 hours of oogenesis and the first three hours of embryonic development took place at 18 degrees. The females which survived under these conditions displayed morphological abnormalities in a variety of adult cuticular structures, characterisitc of cell death during development. In contrast, their male siblings were morphologically normal. Upon prolonged exposure to 29 degrees, da/da females became sterile but continued to lay eggs. Some sexually mosaic progeny from da/da mothers survived even at 25 degrees, but the distribution and development of the female tissue in these mosaics were abnormal. It is suggested that there are multiple functions of the da+ gene during oogenesis, one of which may be required specifically for the subsequent survival of female cells throughout the embryo. In addition to and distinct from its effect during oogenesis, the da mutation acted in both sexes of progeny as a recessive temperature-sensitive lethal mutation with a TSP during the first half of embryonic development.