Establishment of an immortal turkey turbinate cell line suitable for avian metapneumovirus propagation
Establishment of an immortal turkey turbinate cell line suitable for avian metapneumovirus propagation
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DOI：
10.1016/j.virusres.2007.03.028
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发表时间：
2007-07-01
期刊：
影响因子：
5
通讯作者：
Foster, Douglas N.
中科院分区：
文献类型：
--
作者：
Kong, Byung-Whi;Foster, Linda K.;Foster, Douglas N.
直到最近，还没有一个同源的禽细胞基板，可以连续生产高滴度禽偏肺病毒（AMPV），这样的细胞系的发展应该提供一个很好的模型系统，研究AMPV感染。我们已经建立了一个非致瘤性永生火鸡鼻甲细胞系（TT-1）繁殖足够高的AMPV滴度。目前，永生TT-1细胞以每天1.2-1.4群体倍增率连续生长，并在第160代。动力学分析表明，AMPV可以感染和复制TT-1比Vero细胞更迅速，虽然两种细胞类型进行细胞凋亡后感染。非致瘤性逆转录酶阴性TT-1细胞系可作为病毒繁殖的优良同源细胞基质。(C)2007 Elsevier B. V.保留所有权利。
Until recently, there has not been a homologous avian cellular substrate which could continuously produce high titer avian metapneumovirus (AMPV); development of such a cell line should provide an excellent model system for studying AMPV infection. We have established a non-tumorigenic immortal turkey turbinate cell line (TT-1) to propagate sufficiently high AMPV titers. Currently, immortal TT-1 cells are growing continuously at 1.2-1.4 population doublings per day and are at passage 160. Kinetic analysis suggests that AMPV can infect and replicate more rapidly in TT-1 compared to Vero cells, although both cell types undergo apoptosis upon infection. The non-tumorigenic, reverse transcriptase negative TT-1 cell line can serve as an excellent homologous cellular substrate for virus propagation. (C) 2007 Elsevier B.V. All rights reserved.