Analysis of biomarkers characteristic of porcine liver injury-from biomolecular logic gates to an animal model
Analysis of biomarkers characteristic of porcine liver injury-from biomolecular logic gates to an animal model
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DOI：
10.1039/c2an00014h
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Katz, Evgeny
中科院分区：
文献类型：
--
作者：
Halamkova, Lenka;Halamek, Jan;Katz, Evgeny
在创伤性肝损伤动物模型（家猪，Sus scrofa domesticus）的真实样本上，采用生物催化级联分析了两种肝损伤特征生物标志物（丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH））浓度的同时升高。在两种酶生物标志物的存在下进行两步生物催化反应，导致NADH氧化，随后进行光学吸光度测量。一个简单的定性，YES/NO，测试允许区分有和没有肝损伤的动物，概率为92%。这些数据首次展示了将二进制逻辑系统应用于实际生物医学样本的分析。
A biocatalytic cascade for the analysis of the simultaneous increase in the concentration of two biomarkers characteristic of liver injury (alanine transaminase, ALT, and lactate dehydrogenase, LDH) was tested on real samples acquired from an animal model (domestic pigs, Sus scrofa domesticus) suffering from traumatic liver injury. A two-step reaction biocatalyzed in the presence of both enzyme-biomarkers resulted in the oxidation of NADH followed by optical absorbance measurements. A simple qualitative, YES/NO, test allowed for distinction between animals with and without the presence of liver injury with the probability of 92%. These data represent the first demonstration of applying binary logic systems for the analysis of real biomedical samples.