A Novel Endoribonuclease, RNase LS, in Escherichia coli
A Novel Endoribonuclease, RNase LS, in Escherichia coli
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DOI：
10.1534/genetics.104.033290
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发表时间：
2005-01
期刊：
影响因子：
3.3
通讯作者：
Y. Otsuka;T. Yonesaki
中科院分区：
文献类型：
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作者：
Y. Otsuka;T. Yonesaki
噬菌体T4的dmd基因是晚期基因mRNA稳定性所必需的。当该基因突变时，晚期基因由于其mRNA的快速降解而全局沉默。我们以前的工作表明，一种新的大肠杆菌核酸内切酶，RNase LS，是负责快速降解的mRNA。在这项研究中，我们证明了rnlA（以前的yfjN）对于体内和体外的RNA酶LS活性都是必需的。此外，我们还研究了RNase LS在大肠杆菌RNA代谢中的作用。在营养生长条件下培养大肠杆菌细胞。rnlA的突变降低了许多E. colimRNA的稳定性，尽管不同的mRNA的稳定性的突变效果存在差异。此外，我们发现，一个307个核苷酸的片段与23 S rRNA的内部序列积累到高水平的rnlA突变细胞。这些结果有力地表明RNase LS在E. coli以及噬菌体T4。
The dmd gene of bacteriophage T4 is required for the stability of late-gene mRNAs. When this gene is mutated, late genes are globally silenced because of rapid degradation of their mRNAs. Our previous work suggested that a novel Escherichia coli endonuclease, RNase LS, is responsible for the rapid degradation of mRNAs. In this study, we demonstrated that rnlA (formerly yfjN) is essential for RNase LS activity both in vivo and in vitro. In addition, we investigated a role of RNase LS in the RNA metabolism of E. coli cells under vegetative growth conditions. A mutation in rnlA reduced the decay rate of many E. coli mRNAs, although there are differences in the mutational effects on the stabilization of different mRNAs. In addition, we found that a 307-nucleotide fragment with an internal sequence of 23S rRNA accumulated to a high level in rnlA mutant cells. These results strongly suggest that RNase LS plays a role in the RNA metabolism of E. coli as well as phage T4.