ZO-1 and cingulin: tight junction proteins with distinct identities and localizations.
ZO-1 and cingulin: tight junction proteins with distinct identities and localizations.
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ZO-1 和 cingulin：具有不同身份和定位的紧密连接蛋白。
DOI：
10.1152/ajpcell.1989.257.4.c621
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mooseker,MS
中科院分区：
文献类型：
--
作者：
Stevenson,BR;Heintzelman,MB;Anderson,JM;Citi,S;Mooseker,MS
ZO-1 和 cingulin（紧密连接的唯一两种已知成分）在 Madin-Darby 犬肾 (MDCK) 细胞、鸡小肠、大鼠肾远曲小管和肝癌细胞系中的相对定位进行了比较。免疫印迹分析表明，cingulin 和 ZO-1 在免疫学上不相关，并且在结肠中，cingulin 是分子量为 140 kDa 的单一多肽。 MDCK 细胞汇合单层中 cingulin 和 ZO-1 的免疫荧光定位显示出相同的染色模式。然而，亚汇合的 MDCK 细胞显示出两种蛋白质的不同定位。 cingulin 和 ZO-1 均仅在细胞与细胞接触的区域的质膜上发现，但 cingulin 广泛分布在细胞质内，而 ZO-1 显示出更聚集的内部排列。 Cingulin 和 ZO-1 相同地定位于肝癌组织培养 (HTC) 细胞质膜上的细胞-细胞接触部位。在超微结构水平检查的鸡肠中，发现与 cingulin 相关的免疫金颗粒距连接膜的距离大约是与 ZO-1 相关的免疫金颗粒的三倍。
The relative localization of ZO-1 and cingulin, the only two known components of the tight junction, was compared in Madin-Darby canine kidney (MDCK) cells, chicken small intestine, rat kidney distal convoluted tubule, and a hepatoma cell line. Immunoblot analysis demonstrated that cingulin and ZO-1 are immunologically unrelated and that, in the colon, cingulin is a single polypeptide with a molecular mass of 140 kDa. Immunofluorescent localization of cingulin and ZO-1 in confluent monolayers of MDCK cells showed identical staining patterns. However, subconfluent MDCK cells showed distinct localizations of the two proteins. Both cingulin and ZO-1 were found at the plasma membrane only at areas of cell-cell contact, but cingulin was diffusely distributed within the cytoplasm, whereas ZO-1 showed a more clustered internal arrangement. Cingulin and ZO-1 were identically localized at the plasma membrane of hepatoma tissue culture (HTC) cells at sites of cell-cell contact. In chicken intestine examined at the ultrastructural level, immunogold particles associated with cingulin were found approximately three times farther from the junctional membrane than those affiliated with ZO-1.