Pyrrole-hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers
Pyrrole-hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers
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DOI：
10.1016/j.actbio.2010.06.011
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发表时间：
2010-11-01
影响因子：
9.7
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
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作者：
Lee, Jae Young;Schmidt, Christine E.
导电性生物材料的表面修饰已被研究，以改善生物相容性的许多应用，如植入式传感器和微电极阵列。在这项研究中，我们用生物相容性和非细胞粘附透明质酸（HA）电化学涂覆电极，以减少细胞粘附，这可能用于神经假体。为此，合成了吡咯共轭透明质酸（PyHA），并将其电化学涂覆在铂、氧化铟锡和聚苯乙烯磺酸掺杂的聚吡咯电极上。这种PyHA缀合物由(1)吡咯片段组成，允许化合物在导电底物上进行电化学聚合；(2)非粘附性HA，以减少细胞粘附并潜在地减少炎症组织反应。我们的表征结果表明，修饰电极上存在亲水性p（PyHA）层，阻抗测量显示阻抗与未修饰电极在统计学上相同。我们发现，p（PyHA）包被电极最小化成纤维细胞和星形胶质细胞的粘附和迁移至少长达3个月。该涂层在生理溶液中具有3个月的稳定性和抗透明质酸酶降解的稳定性。这些研究表明，这种p（PyHA）涂层有可能用于掩盖导电电极在植入时发生的不良胶质反应。此外，PyHA电化学涂层有可能扩展到其他金属和导电物质的表面改性，如支架和生物传感器。由爱思唯尔有限公司代表Acta Materialia公司出版。
Surface modification of electrically conductive biomaterials has been studied to improve biocompatibility for a number of applications, such as implantable sensors and microelectrode arrays. In this study we electrochemically coated electrodes with biocompatible and non-cell adhesive hyaluronic acid (HA) to reduce cellular adhesion for potential use in neural prostheses. To this end, pyrrole-conjugated hyaluronic acid (PyHA) was synthesized and employed to electrochemically coat platinum, indium-tin oxide and polystyrene sulfonate-doped polypyrrole electrodes. This PyHA conjugate consisted of (1) a pyrrole moiety that allowed the compound to be electrochemically polymerized onto a conductive substrate and (2) non-adhesive HA to minimize cell adhesion and to potentially decrease inflammatory tissue responses. Our characterization results showed the presence of a hydrophilic p(PyHA) layer on the modified electrode, and impedance measurements revealed an impedance that was statistically the same as the unmodified electrode. We found that the p(PyHA)-coated electrodes minimized adhesion and migration of fibroblasts and astrocytes for a minimum of up to 3 months. Also, the coating was stable in physiological solution for 3 months and was stable against enzymatic degradation by hyaluronidase. These studies suggest that this p(PyHA) coating has the potential to be used to mask conducting electrodes from adverse glial responses that occur upon implantation. In addition, electrochemical coating with PyHA could potentially be extended for the surface modification of other metallic and conducting substances, such as stents and biosensors. Published by Elsevier Ltd. on behalf of Acta Materialia Inc.