Expression of Golf in the rat placenta: Possible implication in olfactory receptor transduction
Expression of Golf in the rat placenta: Possible implication in olfactory receptor transduction
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DOI：
10.1016/j.placenta.2004.12.006
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发表时间：
2006-01-01
期刊：
影响因子：
3.8
通讯作者：
Kanayama, N
中科院分区：
文献类型：
--
作者：
Itakura, S;Ohno, K;Kanayama, N
嗅觉受体是G蛋白偶联受体，由哺乳动物中极其庞大且多样的基因家族编码。越来越多的证据表明嗅觉受体广泛分布于许多器官中，提示嗅觉受体不仅识别空气中的气味物质，而且在胚胎的趋化性或器官构建中起重要作用。在这项研究中，我们调查是否嗅觉受体和他们的转导分子，高尔夫球表达在大鼠胎盘。通过RT-PCR，我们确定了11个不同的嗅觉受体基因，这都是第二类的成员，在大鼠胎盘cDNA，我们的结果表明，嗅觉受体基因家族的特定成员可能优先在胎盘中表达。通过蛋白质印迹分析，我们证明了高尔夫蛋白在胎盘中表达，并且其表达水平受到发育调节。我们发现，高尔夫球免疫反应是专门定位于巨细胞滋养层细胞和海绵滋养层细胞。这些发现提出了一种可能性，即嗅觉受体的特定子集可能与高尔夫球偶联，并在巨细胞滋养层和海绵滋养层细胞中发挥作用。(c)2005爱思唯尔有限公司保留所有权利。
Olfactory receptors are G-protein coupled receptors and are encoded by an extremely large and diverse family of genes in mammals. There is increasing evidence that olfactory receptors are widely distributed in many organs, suggesting that olfactory receptors do not only recognize airborne odorants but also play important roles in chemotaxis or organ construction in embryo. In this study, we investigated whether olfactory receptors and their transduction molecule, Golf are expressed in the rat placenta. By RT-PCR, we identified 11 different olfactory receptor genes, which are all members of class II, in the rat placenta cDNAs, and our results suggested that particular members of the olfactory receptor gene family might be preferentially expressed in the placenta. By western blot analysis, we demonstrated that Golf protein is expressed in the placenta and its expression levels are developmentally regulated. We found that Golf immunoreactivity is exclusively localized to giant cell trophoblasts and spongiotrophoblast cells. These findings raised a possibility that a particular subset of olfactory receptors might be coupled with Golf and function in giant cell trophoblasts and spongiotrophoblast cells. (c) 2005 Elsevier Ltd. All rights reserved.