Nuclear and cytoplasmic expression of survivin in 67 surgically resected pancreatic cancer patients
Nuclear and cytoplasmic expression of survivin in 67 surgically resected pancreatic cancer patients
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DOI：
10.1038/sj.bjc.6602632
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发表时间：
2005-06-20
影响因子：
8.8
通讯作者：
Picciocchi, A
中科院分区：
文献类型：
--
作者：
Tonini, G;Vincenzi, B;Picciocchi, A
胰腺癌是最具侵袭性的胃肠道癌症之一，长期存活率不到10%。细胞凋亡途径失调是这种肿瘤发生的一种假定机制。本研究调查了一系列手术切除的胰腺癌患者中细胞凋亡和凋亡相关蛋白的预后作用。所有受胰腺癌影响并在1988年至2003年接受手术切除治疗的患者均被纳入本研究。记录患者的临床资料和肿瘤病理特征。免疫组化法检测Survivin和考克斯-2的表达。TUNEL法检测凋亡细胞。67例手术切除患者的肿瘤标本纳入研究。通过单因素分析，生存率受Survivin过表达的影响。Survivin细胞核过表达与预后相关（P = 0.0009），而胞浆过表达与预后相关（P = 0.0127）。此外，在单变量模型中，凋亡指数是一个统计学显著的预后因素（P = 0.0142）。多因素考克斯回归分析显示，Survivin在细胞核（P = 0.002）和细胞浆（P = 0.040）的过表达均具有预后意义。这是第一个研究报告了胰腺癌细胞核和细胞质Survivin过表达的统计学显著预后相关性。特别是，细胞核Survivin染色高的患者生存期较长，而细胞质Survivin染色高的患者总生存期较短。
Pancreatic cancer is one of the most aggressive gastrointestinal cancer with less than 10% long-term survivors. The apoptotic pathway deregulation is a postulated mechanism of carcinogenesis of this tumour. The present study investigated the prognostic role of apoptosis and apoptosis-involved proteins in a series of surgically resected pancreatic cancer patients. All patients affected by pancreatic adenocarcinoma and treated with surgical resection from 1988 to 2003 were considered for the study. Patients' clinical data and pathological tumour features were recorded. Survivin and Cox-2 expression were evaluated by immunohistochemical staining. Apoptotic cells were identified using the TUNEL method. Tumour specimen of 67 resected patients was included in the study. By univariate analysis, survival was influenced by Survivin overexpression. The nuclear Survivin overexpression was associated with better prognosis ( P = 0.0009), while its cytoplasmic overexpression resulted a negative prognostic factor ( P = 0.0127). Also, the apoptotic index was a statistically significant prognostic factor in a univariate model ( P = 0.0142). By a multivariate Cox regression analysis, both the nuclear ( P = 0.002) and cytoplasmic ( P = 0.040) Survivin overexpression maintained the prognostic statistical value. This is the first study reporting a statistical significant prognostic relevance of nuclear and cytoplasmic Survivin overexpression in pancreatic cancer. In particular, patients with high nuclear Survivin staining showed a longer survival, whereas patients with high cytoplasmic Survivin staining had a shorter overall survival.