THE IGE AND IGG SUBCLASS RESPONSES OF MICE TO 4 HELMINTH-PARASITES
THE IGE AND IGG SUBCLASS RESPONSES OF MICE TO 4 HELMINTH-PARASITES
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DOI：
10.1016/0008-8749(89)90235-9
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发表时间：
1989-03-01
影响因子：
4.3
通讯作者：
SPIEGELBERG, HL
中科院分区：
文献类型：
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作者：
ZAKROFF, SGH;BECK, L;SPIEGELBERG, HL
为了研究IgE的形成是否与IgG亚类相关，用放射免疫法测定了小鼠感染巴西尼波圆线虫（Nippostrongylus brasiliensis, Nbr）、corti Mesocestoides corti、带绦虫（Taenia crassiceps）和旋毛虫（Trichinella spiralis） 4种寄生虫后血清中不同IgG亚型的水平变化以及对corti M.和T. crassiceps抗原提取物的抗体反应。所有四种寄生虫都引起IgE和IgG1血清水平的升高，通常是IgG2a水平的降低。它们也诱导IgM水平升高，但对血清IgG2b、IgG3和IgA水平影响不大。corti抗原提取物的特异性抗体主要为IgG1亚类，而对稻蠹蛾的特异性抗体为IgG1和IgG2a亚类。注射死M. corti诱导所有IgG亚类增加，IgG1和IgG2a抗寄生虫抗体水平相似。皮下感染代替腹腔感染诱导IgG2a水平高于IgG1水平，IgE水平比自然感染低10倍；然而，尽管IgG2a多克隆反应更强，抗寄生虫抗体主要是IgG1亚类。数据表明，四种不同寄生虫的自然感染诱导了伴随的多克隆IgG1和IgE反应。这些体内观察证实了最近的体外研究结果，表明白细胞介素-4诱导脂多糖激活的小鼠B细胞分泌IgG1和IgE，表明这两种同种型的调节是相互关联的。
To investigate whether the formation of IgE is linked in vivo to an IgG subclass, mice were infected with four helminth parasites, Nippostrongylus brasiliensis (Nbr), Mesocestoides corti, Taenia crassiceps and Trichinella spiralis and the changes in the serum levels of the different Ig isotypes as well as the antibody response to M. corti and T. crassiceps antigen extracts were determined by radioimmunoassays. All four parasites induced a concomitant increase of the IgE and IgG1 serum levels and usually a decrease of the IgG2a level. They also induced an increase of the IgM level but had little effect on the IgG2b, IgG3, and IgA serum levels. The specific antibodies to an M. corti antigen extract were mainly of the IgG1 subclass, whereas it was of both IgG1 and IgG2a subclasses to T. crassiceps. Injections of dead M. corti induced an increase of all IgG subclasses and similar levels of IgG1 and IgG2a anti-parasite antibodies. Subcutaneous instead of intraperitoneal infection with T. crassiceps induced higher IgG2a than IgG1 levels and 10-fold lower IgE levels than the natural ip infection; however, despite the greater IgG2a polyclonal response, anti-parasite antibodies were predominantly of the IgG1 subclass. The data demonstrate that natural infection with four different helminth parasites induces a concomitant polyclonal IgG1 and IgE response. These in vivo observations corroborate the recent in vitro findings demonstrating that interleukin-4 induces lipopolysaccharide-activated murine B cells to secrete both IgG1 and IgE, suggesting that the regulation of these two isotypes is linked.