Characterization of rhodamine B hydroxylamide as a highly selective and sensitive fluorescence probe for copper(II)
Characterization of rhodamine B hydroxylamide as a highly selective and sensitive fluorescence probe for copper(II)
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DOI：
10.1016/j.aca.2007.08.025
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发表时间：
2009-01-19
影响因子：
6.2
通讯作者：
Wang, Xiaochun
中科院分区：
文献类型：
--
作者：
Chen, Xinqi;Jia, Jia;Wang, Xiaochun
罗丹明B羟酰胺(1)的特点是高选择性和敏感的Cu2+荧光探针。在优化的条件下，探针仅对Cu2+表现出特异性的吸收和荧光响应。这种显著的特性可以使Cu2+在其他过渡金属离子存在的情况下直接被检测到，并且这种应用已经在人血清中得到证实。还研究并提出了反应机理为1的羟酰胺基团与Cu2+结合，随后Cu2+的络合对酰胺键的水解裂解表现出较高的催化活性，引起荧光团（罗丹明B）的释放，从而恢复吸光度和荧光。在1 ~ 20 μ m范围内，恢复的荧光强度与Cu2+的浓度成正比，Cu2+的检测升/单位为33 nM （k = 3）。本文描述的反应机理可能有助于为其他分析物开发具有可切割活性键的优良光谱探针。(C) 2007 Elsevier B.V.版权所有
Rhodamine B hydroxylamide (1) is characterized as a highly selective and sensitive fluorescence probe for Cu2+. Under the optimized conditions, the probe exhibits specific absorbance-on and fluorescence-on responses to Cu2+ only. This remarkable property may allow Cu2+ to be detected directly in the presence of the other transition metal ions, and such an application has been demonstrated to human serum. The reaction mechanism is also investigated and proposed as that the hydroxylamide group of 1 binds Cu2+, and the subsequent complexation of Cu2+ displays a high catalytic activity for the hydrolytic cleavage of the amide bond, causing the release of fluorophore (rhodamine B) and thereby the retrievement of absorbance and fluorescence. The recovered fluorescence intensity is proportional to the concentration of Cu2+ in the range 1-20 mu M. The detection lit-nit for Cu2+ is 33 nM (k = 3). The reaction mechanism described here may be useful for developing excellent spectroscopic probes with cleavable active bonds for other analytes. (C) 2007 Elsevier B.V. All rights reserved.