First evidence showing that Pepper vein yellows virus P4 protein is a movement protein
First evidence showing that Pepper vein yellows virus P4 protein is a movement protein
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第一个证据表明辣椒静脉黄化病毒 P4 蛋白是一种运动蛋白
DOI：
10.1186/s12866-020-01758-y
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发表时间：
2020-03-30
期刊：
影响因子：
4.2
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Li, Sangsang;Su, Xianyan;Liu, Yong
背景
植物病毒通过胞间连丝（PD）感染新的细胞。为了克服PD障碍，植物病毒已经开发了特异性蛋白质来引导它们的基因组RNA或DNA穿过PD。
结果
本研究对辣椒脉黄病毒P4蛋白的功能进行了初步分析。我们使用五种常用算法的生物信息学分析表明，P4蛋白含有一个跨膜结构域，包含氨基酸残基117-138。P4蛋白的亚细胞定位被发现以PD为靶点，并在壁附近形成小斑点。通过重叠PCR构建的P4缺失突变体或替换突变体失去了在荧光位点内的壁附近产生点状物的功能。P4-YFP融合蛋白在侵染的叶片中可以在细胞间移动，并且P4可以补充黄瓜花叶病毒移动蛋白缺失突变体在细胞间移动。
结论
综合以上结果，我们认为P4蛋白是辣椒脉黄病毒的运动蛋白。
Background
Plant viruses move through plasmodesmata (PD) to infect new cells. To overcome the PD barrier, plant viruses have developed specific protein(s) to guide their genomic RNAs or DNAs to path through the PD.
Results
In the present study, we analyzed the function of Pepper vein yellows virus P4 protein. Our bioinformatic analysis using five commonly used algorithms showed that the P4 protein contains an transmembrane domain, encompassing the amino acid residue 117–138. The subcellular localization of P4 protein was found to target PD and form small punctates near walls. The P4 deletion mutant or the substitution mutant constructed by overlap PCR lost their function to produce punctates near the walls inside the fluorescent loci. The P4-YFP fusion was found to move from cell to cell in infiltrated leaves, and P4 could complement Cucumber mosaic virus movement protein deficiency mutant to move between cells.
Conclusion
Taking together, we consider that the P4 protein is a movement protein of Pepper vein yellows virus.