Mechanotransduction events at the physiological site of touch detection.
Mechanotransduction events at the physiological site of touch detection.
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DOI：
10.7554/elife.84179
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发表时间：
2023-01-06
期刊：
影响因子：
7.7
通讯作者：
Bagriantsev, Sviatoslav N.
中科院分区：
文献类型：
--
作者：
Ziolkowski, Luke H.;Gracheva, Elena O.;Bagriantsev, Sviatoslav N.
关键词：
外周机械感受器的传入神经支配脊椎动物的皮肤，它们通过机械门控离子通道（机械传感器）检测物理触摸。虽然传入终端通常被认为是力传导的主要部位，但该位置的力传导器产生的机械激活（MA）离子电流的功能特性仍然不清楚。到目前为止，尚未获得机械感受器末梢中 MA 电流和机械感应动作电位的直接证据。在这里，我们报告了来自触觉专家鸭嘴皮肤中支配格兰德里（迈斯纳）小体的传入终端的膜片钳记录。我们表明，机械刺激会在机械刺激的动态阶段期间以快速的激活和失活动力学在传入神经中诱发 MA 电流。这些反应触发在终端检测到的传入纤维和小体外部的传入纤维中的快速适应放电。我们的研究结果阐明了机械感受器神经末梢触摸检测的初始电事件。
Afferents of peripheral mechanoreceptors innervate the skin of vertebrates, where they detect physical touch via mechanically gated ion channels (mechanotransducers). While the afferent terminal is generally understood to be the primary site of mechanotransduction, the functional properties of mechanically activated (MA) ionic current generated by mechanotransducers at this location remain obscure. Until now, direct evidence of MA current and mechanically induced action potentials in the mechanoreceptor terminal has not been obtained. Here, we report patch-clamp recordings from the afferent terminal innervating Grandry (Meissner) corpuscles in the bill skin of a tactile specialist duck. We show that mechanical stimulation evokes MA current in the afferent with fast kinetics of activation and inactivation during the dynamic phases of the mechanical stimulus. These responses trigger rapidly adapting firing in the afferent detected at the terminal and in the afferent fiber outside of the corpuscle. Our findings elucidate the initial electrogenic events of touch detection in the mechanoreceptor nerve terminal.