Genetic polymorphism investigation of the Chinese Yi minority using PowerPlexA® Y23 STR amplification system
Genetic polymorphism investigation of the Chinese Yi minority using PowerPlexA® Y23 STR amplification system
复制标题
利用PowerPlex(R) Y23 STR扩增系统对中国彝族遗传多态性进行研究。
DOI：
10.1007/s00414-017-1537-2
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发表时间：
2017-05-01
影响因子：
2.1
通讯作者：
Hou, YiPing
中科院分区：
文献类型：
--
作者：
He, GuangLin;Chen, PengYu;Hou, YiPing
23个Y-STR基因座（DYS576、DYS389 I、DYS389 II、DYS448、DYS19、DYS391、DYS481、DYS549、DYS533、DYS438、DYS437、DYS570、DYS635、DYS390、DYS439、DYS392、DYS393、DYS458、DYS456、DYS643、YGATA-H4、和DYS 385 a/B）在来自中国四川凉山彝族自治州彝族人群的311名无血缘关系的健康男性个体中进行了首次调查。在彝族群体中共发现179个等位基因和297个单倍型。其中285个单倍型是独特的，其余12个单倍型在两个或三个个体中观察到。单倍型区分能力和单倍型多样性分别为0.9550和0.9989。遗传多样性介于0.4550（DYS 437）~ 0.9556（DYS 385 a/B）之间。对彝族和10个亚洲元人群（包括58个个体人群）进行了人群比较。多维标度图和系统发育分析表明，中国彝族的遗传结构是非常不同的10个亚洲元人群中的台湾原住民相比。此外，彝族群体的遗传结构相似性来自地理上接近的人群（宣威汉族）或相似的语言家族群体（泰国人群）。此外，我们的研究还表明，Powerstrom（R）Y23系统在中国彝族人群中具有高度的多态性，并具有较高的法医学鉴别力。在彝族人群中获得的23个Y-STR群体数据丰富了中国民族遗传学信息。
Twenty-three Y-STR loci (DYS576, DYS389I, DYS389 II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS393, DYS458DYS456, DYS643, YGATA-H4, and DYS385a/b) included in the next-generation PowerPlex (R) Y23 System were first investigated in 311 unrelated, healthy male individuals from the Yi minority population residing in the Liangshan Yi Autonomous Prefecture, Sichuan, China. A total of 179 alleles and 297 haplotypes were discovered in the Yi group. In total, 285 haplotypes among them were unique, and the remaining 12 haplotypes were observed in two or three individuals. Haplotype discrimination capacity and haplotype diversity were 0.9550 and 0.9989, respectively. Genetic diversity ranged from 0.4550 (DYS437) to 0.9556 (DYS385a/b). Population comparisons between the Yi minority group and 10 Asian meta-populations comprising 58 individual populations were performed. Both multidimensional scaling plots and phylogenetic analyses demonstrated that the genetic structure of the Chinese Yi ethnicity was extremely different compared to Taiwan indigenous inhabitants among 10 Asian meta-populations. Additionally, the genetic structure resemblance of the Yi group was obtained from a geographically close population (Xuanwei Han) or similar language family groups (Thai populations). Besides, our study has demonstrated that the PowerPlex (R) Y23 System has high polymorphism in a Chinese Yi ethnic population and high discriminatory power for forensic purposes. Population data of the 23 Y-STR obtained from a Yi ethnic population has enriched the Chinese ethnic genetic information.