Coexpression of Methyltransferase Gene dmt50 and Methylene Tetrahydrofolate Reductase Gene Increases Arabidopsis thaliana Dicamba Resistance
Coexpression of Methyltransferase Gene dmt50 and Methylene Tetrahydrofolate Reductase Gene Increases Arabidopsis thaliana Dicamba Resistance
复制标题
甲基转移酶基因dmt50和亚甲基四氢叶酸还原酶基因共表达提高拟南芥麦草畏抗性
DOI：
10.1021/acs.jafc.8b04944
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发表时间：
2019
影响因子：
6.1
通讯作者：
He Jian
中科院分区：
文献类型：
--
作者：
Chen Le;Yao Shigang;Chen Tao;Tao Qin;Xie Xiangting;Xiao Xiang;Ding Derong;He Qin;He Jian
麦草畏是一种广谱、高效的除草剂，是抗除草剂作物工程的优良靶标除草剂。本研究从麦草畏降解菌Rhizorhabdus dicambivoransNdbn-20中克隆了一个新的依赖四氢叶酸（THF）的麦草畏甲基转移酶基因dmt 50。Dmt 50催化甲基从麦草畏转移到THF，产生无药理活性的产物3，6-二氯水杨酸（3，6-DCSA）和5-甲基-THF。Admt 50转基因拟南芥对麦草畏表现出明显的抗性（560 g/ha，正常田间施用量）。然而，Dmt 50去甲基化活性被产物5-甲基-THF抑制。由5，10-亚甲基四氢呋喃还原酶基因mthfr 66编码的Mthfr 66在体外可以解除5-甲基四氢呋喃的抑制作用。与单独表达dmt 50相比，dmt 50和mthfr 66的同时表达进一步提高了转基因A的麦草畏抗性（1120 g/ha）。thaliana.该研究为麦草畏解毒提供了新的基因，并为麦草畏抗性作物的工程改造提供了策略。
Dicamba, a broad-spectrum and highly efficient herbicide, is an excellent target herbicide for the engineering of herbicide-resistant crops. In this study, a new tetrahydrofolate (THF)-dependent dicamba methyltransferase gene,dmt50, was cloned from the dicamba-degrading strainRhizorhabdus dicambivoransNdbn-20. Dmt50 catalyzed the methyl transfer from dicamba to THF, generating the herbicidally inactive product 3,6-dichlorosalicylic acid (3,6-DCSA) and 5-methyl-THF. Admt50transgenicArabidopsis thalianaclearly showed dicamba resistance (560 g/ha, the normal field application rate). However, Dmt50 demethylation activity was inhibited by the product 5-methyl-THF. Mthfr66, encoded by the 5,10-methylene-THF reductase genemthfr66could relieve the inhibition by removing 5-methyl-THF in vitro. Compared with expression ofdmt50alone, simultaneous expression ofdmt50andmthfr66further improved the dicamba resistance (1120 g/ha) of transgenicA. thaliana. This study provides new genes for dicamba detoxification and a strategy for the engineering of dicamba-resistant crops.