Migration of vascular smooth muscle cells induced by sphingosine 1-phosphate and related lipids: Potential role in the angiogenic response
Migration of vascular smooth muscle cells induced by sphingosine 1-phosphate and related lipids: Potential role in the angiogenic response
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DOI：
10.1006/excr.2002.5472
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发表时间：
2002-04-01
影响因子：
3.7
通讯作者：
English, D
中科院分区：
文献类型：
--
作者：
Boguslawski, G;Grogg, JR;English, D
1-磷酸鞘氨醇（SPP）、鞘氨醇磷酸胆碱和溶血磷脂酸等生物活性脂质对内皮细胞（EC）的迁移和EC单层的完整性均有影响，因此在血管生成中起着重要作用。在这里，我们表明极低浓度的血清和纳摩尔浓度的这些生物活性脂质刺激人主动脉平滑肌细胞（SMC）的迁移。然而，在促进EC迁移和增强EC单层完整性的最有效剂量下，血清和SPP可有效抑制SMC迁移; SPP还可阻断蛋白质生长因子诱导的迁移。用SPP处理SMCs诱导了与PDGF受体对应的175 - 185-kDa蛋白的瞬时磷酸化，表明该受体的反式激活。SPP和相关脂质可能通过协调两者的迁移在血管生成中发挥关键作用。内皮细胞和血管平滑肌细胞响应这些生物活性脂质信使的变化梯度。(C)2002 Elsevier Science（美国）。
The bioactive lipids sphingosine 1-phosphate (SPP), sphingosylphosphorylcholine, and lysophosphatidic acid play an important role in angiogenesis as a result of their effects on both the migration of endothelial cells (ECs) and the integrity of EC monolayers. Here we show that extremely low concentrations of serum and nanomolar concentrations of these biologically active lipids stimulate migration of human aortic smooth muscle cells (SMCs). However, at dosages most effective in promoting EC migration and in enhancing EC monolayer integrity, serum and SPP potently inhibited SMC migration; SPP also blocked the migration induced by protein growth factors. Treatment of SMCs with SPP induced transient phosphorylation of a 175- to 185-kDa protein corresponding to the PDGF receptor, indicating transactivation of this receptor. SPP and related lipids may play a key role in angiogenesis by coordinating the migration of both. endothelial cells and vascular smooth muscle cells in response to the changing gradients of these bioactive lipid messengers. (C) 2002 Elsevier Science (USA).