Apigenin, a Natural Flavonoid, Attenuates EAE Severity Through the Modulation of Dendritic Cell and Other Immune Cell Functions.
Apigenin, a Natural Flavonoid, Attenuates EAE Severity Through the Modulation of Dendritic Cell and Other Immune Cell Functions.
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DOI：
10.1007/s11481-015-9617-x
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Khan ZK
中科院分区：
文献类型：
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作者：
Ginwala R;McTish E;Raman C;Singh N;Nagarkatti M;Nagarkatti P;Sagar D;Jain P;Khan ZK
芹菜素是一种天然类黄酮，存在于多种植物、水果、蔬菜、草药和香料中，已知具有抗氧化和抗炎特性，这些特性在几个世纪以来作为治疗哮喘、失眠、帕金森病、神经痛和带状疱疹的药物方法使用中是显而易见的。然而，关于其对免疫细胞，特别是树突状细胞（DC）的作用的信息相当缺乏，树突状细胞（DC）在免疫特化位置如中枢神经系统（CNS）中维持免疫原性和致耐受性免疫应答之间的关键平衡。在本文中，我们研究了芹菜素在恢复免疫功能和减少神经炎症方面的抗炎特性。在体内，用芹菜素治疗后，在多发性硬化症的C57 BL/6（进行性）和SJL/J（复发缓解型）小鼠模型中观察到实验性自身免疫性脑脊髓炎（EAE）进展和复发的严重程度显著降低。芹菜素处理的EAE小鼠显示，与未处理的EAE小鼠相比，脾DC上α4整联蛋白和CLEC 12 A的表达降低，外周中免疫细胞的保留增加。因此，这与免疫组织化学结果相关，即CNS中免疫细胞浸润减少和脱髓鞘减少。这些结果表明芹菜素对由DC刺激的致病性T细胞进入CNS引起的神经变性作用的保护作用，从而暗示了神经炎性疾病的潜在治疗。
Apigenin, a natural flavonoid, found in several plants, fruits, vegetables, herbs, and spices, is known to have anti-oxidant and anti-inflammatory properties that are evident in the use of these substances for centuries as medicinal approaches to treat asthma, insomnia, Parkinson’s disease, neuralgia, and shingles. However, there is a considerable dearth of information regarding its effect on immune cells, especially dendritic cells (DC) that maintain the critical balance between an immunogenic and tolerogenic immune response, in an immunospecialized location like the central nervous system (CNS). In this paper we looked at the anti-inflammatory properties of Apigenin in restoration of immune function and the resultant decrease in neuroinflammation. In vivo, a significant reduction in severity of experimental autoimmune encephalomyelitis (EAE) progression and relapse was observed in C57BL/6 (progressive) and SJL/J (relapse-remitting) mouse models of multiple sclerosis upon treatment with Apigenin. Apigenin treated EAE mice show decreased expression of α4 integrin and CLEC12A on splenic DCs and an increased retention of immune cells in the periphery compared to untreated EAE mice. This correlated consequently with immunohistochemistry findings of decreased immune cell infiltration and reduced demyelination in the CNS. These results indicate a protective role of Apigenin against the neurodegenerative effects resulting from the entry of DC stimulated pathogenic T cells into the CNS thus implicating a potential therapy for neuroinflammatory disease.