Isolation of urinary exosomes from animal models to unravel noninvasive disease biomarkers.
Isolation of urinary exosomes from animal models to unravel noninvasive disease biomarkers.
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DOI：
10.1007/978-1-61779-959-4_21
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Falcon-Perez, Juan M
中科院分区：
文献类型：
--
作者：
Conde-Vancells, Javier;Falcon-Perez, Juan M
在过去的几年里，疾病生物标志物的发现已经高度发展，由于高通量技术，如蛋白质组学的应用。然而，由于样品中一些蛋白质的复杂性和丰度提高，亚细胞区室的分析已被揭示为基本的，以确定代表性不足的临床相关蛋白质。在这个意义上，存在于不同体液中的包括外来体的细胞外微泡构成了鉴定疾病生物标志物的合适且方便的亚细胞区室。另一方面，动物模型提供了许多优于人类样本的优势，以加速候选生物标志物的鉴定。在这一章中，我们提供了一个详细的方法来纯化和分析尿外泌体，可以应用于不同的疾病，有良好的动物模型的研究。
In the last years, disease biomarker discovery has highly evolved thanks to the application of high--throughput technologies such as proteomics. However, due to the elevated complexity and abundance of some of the proteins in the samples the analysis of subcellular compartments has been revealed to be fundamental in order to identify underrepresented clinically relevant proteins. In this sense, extracellular microvesicles including exosomes that are present in different body fluids constitute a suitable and convenient subcellular compartment to identify disease biomarkers. On the other hand, animal models offer numerous advantages over human samples in order to accelerate the identification of candidate biomarkers. In this chapter we provide a detailed methodology to purify and analyze urinary exosomes that can be applied to the study of different diseases that have good animal models.