Apigenin induces apoptosis in Hep G2 cells:: Possible role of TNF-α and IFN-γ
Apigenin induces apoptosis in Hep G2 cells:: Possible role of TNF-α and IFN-γ
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DOI：
10.1016/j.tox.2005.09.019
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发表时间：
2006-01-16
期刊：
影响因子：
4.5
通讯作者：
Sultana, S
中科院分区：
文献类型：
--
作者：
Khan, TH;Sultana, S
黄酮类化合物是一种具有生物活性的植物性食品成分，对多种慢性疾病具有潜在的化学预防作用。芹菜素是一种常见的膳食类黄酮，大量存在于水果和蔬菜中，被认为具有预防和治疗各种癌症的潜力。在目前的研究中。我们研究了芹菜素（25 μ M和50 μ M）对人肝母细胞瘤来源细胞系Hep G2细胞凋亡的调控作用。芹菜素在tnf - α、ifn - γ释放和诱导半胱天冬酶活性方面诱导程序细胞死亡。芹菜素预处理组tnf - α和ifn - γ水平；与对照组相比，显著且剂量依赖性地升高（28-39%和66-85%）。（208-336%和579-1088%）。芹菜素处理显著诱导caspase-3、-7、-10和caspase-9活性（160-209%和203-270%），呈剂量依赖性。对半胱天冬酶、tnf - α和ifn - γ过程的影响介导了芹菜素诱导细胞凋亡的可能机制。2005爱思唯尔爱尔兰有限公司版权所有。
Flavonoids are one of the biologically active plant food constituents, possessing potential chemopreventive properties against a wide variety of chronic diseases. Apigenin, a common dietary flavonoid abundantly present in fruits and vegetables is believed to possess preventive and therapeutic potential against various cancers. In the present study. we have evaluated regulation of apoptotic cell death by apigenin (25 and 50 mu M) in human hepatoblastoma derived cell line Hep G2. Apigenin-induced programme cell death in terms of TNF-alpha, IFN-gamma release and induction of caspases activity. TNF-alpha and IFN-gamma levels in apigenin-pretreated groups; were significantly and dose dependently elevated as compared to the control values (28-39% and 66-85%). (208-336% and 579-1088%), respectively. Treatment of apigenin significantly induced caspase-3, -7, -10 and caspase-9 activity (160-209% and 203-270%) in a dose-dependent manner. The effects on caspases, TNF-alpha, and IFN-gamma processes mediate the plausible mechanism of apoptosis induction of apigenin. (c) 2005 Elsevier Ireland Ltd. All rights reserved.