Recombination in lineage 1, 3, 5 and 8 of porcine reproductive and respiratory syndrome viruses in China
Recombination in lineage 1, 3, 5 and 8 of porcine reproductive and respiratory syndrome viruses in China
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DOI：
10.1016/j.meegid.2018.12.006
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发表时间：
2019-03-01
影响因子：
3.2
通讯作者：
Yang, Xiaoyan
中科院分区：
文献类型：
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作者：
Liu, Jiankui;Wei, Chunhua;Yang, Xiaoyan
猪繁殖与呼吸综合征（Porcine Reproductive and Respiratory Syndrome，PARS）是一种重要的病毒性猪病，给我国养猪业造成了巨大的经济损失。目前，根据分类系统，2型PRRSV（北美型）的4个主要谱系：谱系1（NADC 30-like）、3（QYYZ-like）、5.1（VR 2332-like）和8.7（JXA 1-like）在中国流行，这引起了人们对病毒重组潜力的担忧。在本研究中，2017年从中国福建省妊娠母猪流产率（25%）中分离到一种名为FJLIUY-2017的新的PRRSV变异株。为了进一步了解这种新型病毒株，我们对FJLIUY-2017的完整基因组进行了表征。FJLIUY-2017与GenBank中的PARS序列比较，证实FJLIUY-2017与PRRSV没有亲缘关系（< 92%），但FJLIUY-2017属于NADC 30-like PRRSV。FJLIUY-2017全长15017个核苷酸（不包括poly（A）尾），与JXA 1同源性为86.2- 86.6（JXA 1、TJ和FJYR），88.9-90.6%伴NADC 30样PRRSV（NADC 30、FJZ 03和CHsx 1401），VR 2332样（VR 2332、RespPRRS MLV和BJ-4）为86.4-86.5%，LV（欧洲型）仅为60.8%。重组分析揭示了基因组结构（ORF 3，ORF 4和ORF 7）和非结构（Nsp 1，Nsp 2，Nsp 6，Nsp 9，Nsp 11和Nsp 12）区域的基因组断裂点，有证据表明谱系1，3，5.1和8.7之间发生了重组事件。总的来说，我们的研究结果提供了进一步的确认，PRRSV是易于进行重组事件。因此，对我国PRRSV的流行进行监测，制定有效的控制策略是至关重要的。
Porcine reproductive and respiratory syndrome (PARS) is one of the most important viral swine diseases, resulting in immense economic losses in Chinese pig industry. Currently, four major lineages: lineage 1 (NADC30-like), 3 (QYYZ-like), 5.1 (VR2332-like) and 8.7 (JXA1-like) of type 2 PRRSV (North American type) have been circulating in China based on classification system, which have caused concern about the potential of virus recombination. In the present study, a novel variant of PRRSV strain named FJLIUY-2017 was isolated from abortion rate (25%) in pregnant gilts in Fujian Province in China in 2017. To further our knowledge about the novel virus strain, we characterized the complete genome of FJLIUY-2017. Comparison to PARS sequences in GenBank confirmed the absence of close relatives (< 92%), but indicated FJLIUY-2017 belonged to NADC30-like PRRSV. The full length of FJLIUY-2017 was determined to be 15017 nucleotides (nt), excluding the poly(A) tail, shared 86.2-86.6% identity with JXA1-like strains (JXA1, TJ and FJYR), 88.9-90.6% with NADC30-like PRRSVs (NADC30, FJZ03 and CHsx1401), 86.4-86.5% with VR2332-like (VR2332, RespPRRS MLV and BJ-4) and only 60.8% with LV (European type). Recombination analyses revealed genomic breakpoints in structural (ORF3, ORF4 and ORF7) and nonstructural (Nsp1, Nsp2, Nsp6, Nsp9, Nsp11 and Nsp12) regions of the genomes with evidence for recombination events between lineages 1, 3, 5.1 and 8.7. Taken altogether, the results of our study provide further confirmation that PRRSV is prone to undergo recombination events. Thus, it is critical to monitor PRRSV evolution in China and establish an effective strategy for the control of PRRS.