Effects of surfactants on the properties of PLGA nanoparticles
Effects of surfactants on the properties of PLGA nanoparticles
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DOI：
10.1002/jbm.a.34040
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发表时间：
2012-08-01
影响因子：
4.9
通讯作者：
Nguyen, Kytai T.
中科院分区：
文献类型：
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作者：
Menon, Jyothi U.;Kona, Soujanya;Nguyen, Kytai T.
本研究的目的是调查的物理特性的聚（D，L-乳酸-共-乙醇酸）（PLGA）纳米粒子（NPs）涂有两种表面活性剂，普朗尼克或常用的聚乙烯醇（PVA），并确定其在体外的效率作为药物载体的癌症治疗。游离表面活性剂细胞毒性结果表明，Pluronic F127（PF 127）在所测试的Pluronic中是最具细胞相容性的，因此选择用于进一步研究的PLGA NP涂层。使用多柔比星（DOX）作为药物模型的释放研究显示，DOX从PVA-和PF 127-涂覆的PLGA NP（分别为PLGA-PVA和PLGA-PF 127）持续释放超过28天。此外，当暴露于两种类型的NP时，人真皮成纤维细胞和人主动脉平滑肌细胞存活率没有显著差异。细胞摄取研究表明，对于前列腺癌和乳腺癌细胞，两种纳米颗粒类型的摄取均具有剂量依赖性。然而，这些癌细胞比PLGA-PVA NP内化更多的PLGA-PF 127 NP。此外，研究表明，载药的PLGA-PF 127 NPs不仅比载药的PLGA-PVA NPs杀死更多的癌细胞，而且在再次暴露时克服了LNCaP、MDA-MB-231和MDA-MB-468癌细胞的耐药性。这些结果表明，PLGA-PF 127纳米颗粒可以形成一个有前途的系统，不仅可以递送抗癌药物，而且还可以克服大多数癌细胞中普遍存在的耐药性。(C)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part A，2012。
The objective of this study was to investigate the physical characteristics of poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) coated with two surfactants, Pluronic or the commonly used polyvinyl alcohol (PVA); and determine their in vitro efficiency as drug carriers for cancer therapy. Free surfactant cytotoxicity results indicated that Pluronic F127 (PF127) was most cytocompatible among the Pluronics tested and hence chosen for coating PLGA NPs for further studies. Release studies using doxorubicin (DOX) as a drug model showed sustained release of DOX from both PVA- and PF127-coated PLGA NPs (PLGA-PVA and PLGA-PF127, respectively) over 28 days. Further, there was no significant difference in human dermal fibroblasts and human aortic smooth muscle cell survival when exposed to both types of NPs. Cellular uptake studies demonstrated that uptake of both nanoparticle types was dose-dependent for both prostate and breast cancer cells. However, these cancer cells internalized more PLGA-PF127 NPs than PLGA-PVA NPs. Moreover, studies showed that drug-loaded PLGA-PF127 NPs not only killed more cancer cells than drug-loaded PLGA-PVA NPs, but also overcame drug resistance in LNCaP, MDA-MB-231, and MDA-MB-468 cancer cells on re-exposure. These results indicate that PLGA-PF127 NPs can form a promising system that not only delivers anti-cancer drugs, but also overcomes drug resistance, which is prevalent in most cancer cells. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.