The effect of early auditory deprivation on the age-dependent expression pattern of NR2B mRNA in rat auditory cortex
The effect of early auditory deprivation on the age-dependent expression pattern of NR2B mRNA in rat auditory cortex
复制标题
早期听觉剥夺对大鼠听觉皮层NR2B mRNA年龄依赖性表达模式的影响
DOI：
10.1016/j.brainres.2006.06.056
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发表时间：
2006-09-19
期刊：
影响因子：
2.9
通讯作者：
Sun, Xinde
中科院分区：
文献类型：
--
作者：
Bi, Caixia;Cui, Yilei;Sun, Xinde
NMDA受体已被充分证明与神经元可塑性有关。为了了解NR2B亚型NMDA受体在听觉功能发育中的作用，本研究研究了早期听觉剥夺对大鼠听觉皮层（AC） NR2B mRNA表达的影响。正常大鼠NR2B mRNA表达在出生时最高（出生后第1天[P1]），成年后迅速下降至低水平。然而，在大鼠听觉发育关键期（出生后2 ~ 3周），NR2B在出生后第21天出现了短暂的表达高峰（P21）。听觉剥夺大鼠NR2B mRNA表达从出生到成年的总体下降趋势与正常组相似，而P15至P27的表达水平明显低于正常，P21的瞬时峰值消失。两组均检测了NR2B mRNA阳性神经元在AC各层及背侧、内侧和腹侧亚区的分布规律。正常组和剥夺组对AC NR2B mRNA的空间表达无显著影响。我们的研究结果表明，早期听觉剥夺降低了大鼠听觉发育关键时期AC中NR2B mRNA的表达水平，提示NR2B在大鼠听觉功能的发育可塑性中起重要作用。(c) 2006 Elsevier B.V.版权所有
NMDA receptors have been well shown to be involved in neuronal plasticity. in order to understand the role of NR2B subtype NMDA receptors in auditory function development, the present study investigated the effect of early auditory deprivation on the expression of NR2B mRNA in rat auditory cortex (AC) during postnatal development. For normal rats, the NR2B mRNA expression was highest at birth (postnatal day 1 [P1]) and declined rapidly to low level during adulthood. However, during the critical period of rat auditory development (two to three weeks after birth), there was a transient NR2B expression peak on postnatal day 21 (P21). For the auditory-deprived rats, the general declining trend of NR2B mRNA expression from birth to adult was similar to that observed in the normal group, whereas the expression level from P15 to P27 was significantly lower than normal and the transient peak on P21 disappeared. in both groups, the distribution pattern of NR2B mRNA-positive neurons was also examined in various layers and dorsal, medial and ventral subdistricts of AC. There is no significant effect on the spatial expression of the NR2B mRNA in the AC between normal and deprived group. our results indicated that the early auditory deprivation decreased the expression levels of NR2B mRNA in AC during the critical period of rat auditory development, suggesting that NR2B plays an important role in the developmental plasticity of auditory function in rats. (c) 2006 Elsevier B.V. All rights reserved.