Tartrate-resistant acid phosphate activity as osteoclastic marker: Sensitivity of cytochemical assessment and serum assay in comparison with standardized osteoclast histomorphometry
Tartrate-resistant acid phosphate activity as osteoclastic marker: Sensitivity of cytochemical assessment and serum assay in comparison with standardized osteoclast histomorphometry
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DOI：
10.1007/bf01623458
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发表时间：
1997-01-01
影响因子：
4
通讯作者：
Bonucci, E
中科院分区：
文献类型：
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作者：
Ballanti, P;Minisola, S;Bonucci, E
抗酒石酸酸性磷酸酶（TRAP）活性被认为是破骨细胞的重要细胞化学标志物;其血清浓度被用作破骨细胞功能和骨吸收程度的生化标志物。本研究进行了评估的灵敏度TRAP活性作为细胞化学标记物在组织切片和血清中的生化标记物与破骨细胞的标准化组织形态计量学变量相比。为此，我们研究了24例患者（21例女性，3例男性; 60 +/- 17岁）患有各种代谢性骨病。采用标准化组织形态计量学方法对髂嵴活检组织中的破骨细胞表面（OcS/BS）和破骨细胞数量（OcN/BS）进行评价。在TRAP细胞化学活性的基础上，测量TRAP阳性破骨细胞表面（TRAP+OcS/BS）和数量（TRAP+OcN/BS）。TRAP阳性细胞邻近骨，并显示一个细胞核或没有细胞核，在所有的平面上的切片被列入计数作为破骨细胞。分光光度法测定血清TRAP活性。OcS/BS和OcN/BS的值分别远低于TRAP+OcS/BS（-50%）和TRAP+OcN/BS（-60%）。OcS/BS与TRAP+OcS/BS、OcN/BS与TRAPS、OcN/BS之间的相关性非常显著。血清TRAP与OcS/BS、OcN/BS及TRAP+OcN/BS显著相关。然而，这些相关性相当低。此外，血清TRAP与TRAP+OcS/BS不相关。从这些结果中，可以得出结论，虽然TRAP活性被确认为用于鉴定破骨细胞的有效细胞化学标志物，但血清TRAP活性是弱敏感性的破骨细胞标志物。这可能是由于已知的因素，如酶的合成不是破骨细胞所独有的，酶不稳定性和血清中存在抑制剂。单核破骨细胞不显着影响血清酶水平。
Tartrate-resistant acid phosphatase (TRAP) activity is regarded as an important cytochemical marker of osteoclasts; its concentration in serum is utilized as a biochemical marker of osteoclast function and degree of bone resorption. This study was carried out to assess the sensitivity of TRAP activity both as a cytochemical marker in histological sections and as a biochemical marker in serum in comparison with the standardized histomorphometric variables of osteoclasts. To this end we investigated 24 patients (21 women, 3 men; 60 +/- 17 years of age) affected with various metabolic bone diseases. Osteoclast surface (OcS/BS) and osteoclast number (OcN/BS) were evaluated by standardized histomorphometry in iliac crest biopsies. On the basis of TRAP cytochemical activity, TRAP-positive osteoclast surface (TRAP+OcS/BS) and number (TRAP+OcN/BS) were measured. TRAP-positive cells adjacent to bone and showing one nucleus or no nuclei at all in the plane of section were included in the counts as osteoclasts. Serum TRAP activity was determined by spectrophotometric assay. Values of OcS/BS and OcN/BS were much lower than those of TRAP+OcS/BS (-50%) and TRAP+OcN/BS (-60%), respectively. Correlations between OcS/BS and TRAP+OcS/BS, and between OcN/BS and TRAPS OcN/BS, were highly significant. Serum TRAP was significantly correlated with OcS/BS, OcN/BS, and TRAP+OcN/BS. These correlations, however, were rather low. Moreover, serum TRAP did not correlate with TRAP+OcS/BS. From these results, the conclusion can be drawn that while TRAP activity is confirmed as a valid cytochemical marker for identification of osteoclasts, serum TRAP activity is an osteoclastic marker of weak sensitivity. This may be due to known factors, such as synthesis of the enzyme not being unique to osteoclasts, enzyme instability, and the presence of inhibitors in serum. Mononucleated osteoclasts do not significantly influence the serum enzyme levels.