Migration of Plasmodium sporozoites through cells before infection
Migration of Plasmodium sporozoites through cells before infection
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DOI：
10.1126/science.291.5501.141
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发表时间：
2001-01-05
期刊：
影响因子：
56.9
通讯作者：
Rodríguez, A
中科院分区：
文献类型：
--
作者：
Mota, MM;Pradel, G;Rodríguez, A
细胞内细菌和寄生虫通常通过在入侵病原体周围形成内化空泡来入侵宿主细胞。疟原虫子孢子是由蚊子传播的疟疾寄生虫的感染阶段，具有进入细胞的替代机制。我们观察到宿主细胞的质膜破裂，随后迅速修复。这种进入方式不会导致子孢子周围形成空泡，随后寄生虫离开宿主细胞。子孢子穿过几个细胞的胞质溶胶，然后通过形成寄生虫空泡侵入肝细胞，在其中它们进入下一个感染阶段。在哺乳动物宿主中，子孢子通过几个细胞的迁移似乎是完成生命周期所必需的。
Intracellular bacteria and parasites typically invade host cells through the formation of an internalization vacuole around the invading pathogen. Plasmodium sporozoites, the infective stage of the malaria parasite transmitted by mosquitoes, have an alternative mechanism to enter cells. We observed breaching of the plasma membrane of the host cell followed by rapid repair. This mode of entry did not result in the formation of a vacuole around the sporozoite, and was followed by exit of the parasite from the host cell. Sporozoites traversed the cytosol of several cells before invading a hepatocyte by formation of a parasitophorous vacuole, in which they developed into the next infective stage. Sporozoite migration through several cells in the mammalian host appears to be essential for the completion of the life cycle.