ERF protein JERF1 that transcriptionally modulates the expression of abscisic acid biosynthesis-related gene enhances the tolerance under salinity and cold in tobacco
ERF protein JERF1 that transcriptionally modulates the expression of abscisic acid biosynthesis-related gene enhances the tolerance under salinity and cold in tobacco
复制标题
ERF蛋白JERF1转录调节脱落酸生物合成相关基因的表达增强烟草对盐和寒冷的耐受性
DOI：
10.1007/s00425-007-0528-9
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发表时间：
2007-09-01
期刊：
影响因子：
4.3
通讯作者：
Huang，Rongfeng
中科院分区：
文献类型：
--
作者：
Wu，Lijun;Chen，Xiaoliang;Huang，Rongfeng
越来越多的证据表明，乙烯响应因子（ERF）蛋白调节多种生物和非生物胁迫反应，以及植物的发育。先前，我们证明了编码ERF转录激活因子的jerf1可以被乙烯、MeJA、ABA和NaCl诱导，这表明它可能在多种胁迫反应中起调控作用。在本文中，我们报道了在烟草中表达jerf1通过在叶片液泡中积累钠和稳定质膜，分别提高甘露醇处理下的种子萌发率和对高盐和低温的耐受性，并通过未知的机制显著促进烟草根和叶片在盐和低温下的生长。有证据表明，JERF1与GCC-box、DRE和CE1等多作用元件相互作用，激活胁迫相关基因的表达，支持了JERF1可能以aba依赖和aba不依赖的方式参与植物的多种胁迫反应。更重要的是，我们发现在烟草中表达jerf1可以通过转录调控ABA生物合成相关基因sdr的表达，从而导致ABA含量的增加。综上所述，JERF1与多作用元件相互作用，激活胁迫响应基因和ABA生物合成相关基因的表达，从而引起ABA生物合成，最终增强烟草在高盐低温条件下的耐受性和生长能力。
Increasing evidences indicate that ethylene responsive factor (ERF) proteins regulate a variety of biotic and abiotic stress responses, and plant development as well. Previously we demonstrated thatJERF1, encoding an ERF transcriptional activator, is inducible by ethylene, MeJA, ABA, and NaCl, suggesting its possible regulation in multiple stress responses. In the present paper, we report that expressingJERF1in tobacco increases the seed germination under mannitol treatment, and enhances the tolerance to high salinity and low temperature, through accumulating sodium in vacuole of leaves and stabilizing the plasma membrane, respectively, and significantly increases the growth of tobacco roots and leaves under salinity and low temperature through an unknown mechanism. The evidence that JERF1 interacts with multiplecis-acting elements, such as GCC-box, DRE, and CE1, to activate the expression of stress-related genes, supports the possible involvement of JERF1 in multiple plant stress responses with ABA-dependent and ABA-independent manner. More importantly, we reveal that expressingJERF1in tobacco transcriptionally regulates the expression of ABA biosynthesis-related geneNtSDR, resulting in the increase of the ABA content. Together, our results indicate that JERF1 interacts with multiplecis-acting elements and activates the expression of stress responsive and ABA biosynthesis-related genes, consequently causing ABA biosynthesis, and ultimately enhancing tobacco tolerance and growth under high salinity and low temperature.