Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer
Urine miR-21-5p and miR-200c-3p as potential non-invasive biomarkers in patients with prostate cancer
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DOI：
10.5152/tud.2019.19163
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发表时间：
2020-01-01
影响因子：
1.3
通讯作者：
Haryana, Sofia
中科院分区：
文献类型：
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作者：
Danarto, Raden;Astuti, Indwiani;Haryana, Sofia
目的：为探讨miR-21- 5 p和miR-200 c-3 p在前列腺癌（PCa）患者尿液中的表达及其作为生物标志物的可能性，收集80例前列腺癌患者尿液标本，其中良性前列腺增生（BPH）20例，前列腺癌（PCa）60例。使用miRCURY外泌体分离试剂盒（Miracqon，丹麦）进行外泌体分离，使用miRCURY RNA分离试剂盒-生物流体试剂盒（Miracqon，丹麦）提取总RNA，并使用通用cDNA合成试剂盒（Miracqon，丹麦）合成互补DNA（cDNA）。使用qPCR CFX 96热循环仪（Bio-Rad）进行基因表达的定量聚合酶链反应（qPCR）分析。结果：与BPH组相比，非转移性PCa组和转移性PCa组中miR-21的过度表达具有统计学显著性，p值分别为0.001和0.018。与转移性PCa组相比，非转移性PCa也具有统计学显著性，p值为0.037。与BPH组相比，非转移性PCa组和转移性PCa组中miR-200 c的表达均较低，差异有统计学意义（p值分别为0.001和0.001）。结论：本研究中发现的miR-21过表达可能是PCa患者潜在的非侵入性诊断工具。尽管我们的研究取得了显著的结果，但由于表观遗传变异，尿液样本中micro-RNA的使用可能会有所不同。需要更大人群的进一步研究来研究miR-21和miR-200 c作为PCa生物标志物的作用。
Objective: To evaluate the miR-21-5p and miR-200c-3p expressions in the urine of patients with prostate cancer (PCa) and to investigate their potential as biomarkers.Material and methods: The urine samples collected from 80 patients, including 20 patients diagnosed with benign prostate hyperplasia (BPH) and 60 patients diagnosed with PCa, were examined. The exosome isolation was performed using the miRCURY exosome isolation kit (Exiqon, Denmark), total RNA was extracted using the miRCURY RNA Isolation Kit-Biofluid kit (Exiqon, Denmark), and complementary DNA (cDNA) was synthesized using the Universal cDNA Synthesis kit (Exiqon, Denmark). A quantitative polymerase chain reaction (qPCR) analysis of gene expression was performed using the qPCR CFX 96 Thermocycler (Bio-Rad). All the procedures followed the manufacturer's recommendations.Results: The overexpressions of miR-21 in the non-metastatic PCa and metastatic PCa group compared to the BPH group were statistically significant with a p-value of 0.001 and 0.018, respectively. The non-metastatic PCa compared to the metastatic PCa group was also statistically significant with a p-value of 0.037. The under expressions of miR-200c in the non-metastatic PCa and metastatic PCa group compared to the BPH group are statistically significant with a p-value of 0.001 and 0.001, respectively.Conclusion: The overexpressions of miR-21 found in this study could be a potential non-invasive diagnostic tool for patients with PCa. Despite the significant results in our study, the use of micro-RNA in urine samples may vary due to epigenetic variation. Further studies with larger populations are required to investigate the role of miR-21 and miR-200c as biomarkers in PCa.