Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species
Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species
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DOI：
10.1016/j.febslet.2013.08.029
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发表时间：
2013-11-01
期刊：
影响因子：
3.5
通讯作者：
Stalbrand, Henrik
中科院分区：
文献类型：
--
作者：
Reddy, Sumitha K.;Rosengren, Anna;Stalbrand, Henrik
糖苷水解酶家族2的系统发育分析包括曲霉属序列和来自其他生物的表征的β-甘露糖苷酶，将推定的曲霉属β-甘露糖苷酶聚类为两个不同的进化枝（A和B）。曲霉属物种在两个分支中的每一个中具有至少一个副枝。看来进化枝A成员是细胞外的，而进化枝B成员是细胞内的。对尼日尔曲霉（进化枝A）的MndA和构巢曲霉（进化枝B）的MndB的底物特异性分析表明，与MndA相反，MndB不水解聚合甘露聚糖，并且可能已经进化为水解小的未支化的β-甘露糖苷如甘露二糖。MndB的3D模型提供了进一步的见解。(C)2013年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised beta-mannosidases from other organisms, clusters putative Aspergillus beta-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched beta-mannosides like mannobiose. A 3D-model of MndB provides further insight. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.