ESTABLISHMENT AND CHARACTERIZATION OF 3 TRANSPLANTABLE EBV-CONTAINING NASOPHARYNGEAL CARCINOMAS
ESTABLISHMENT AND CHARACTERIZATION OF 3 TRANSPLANTABLE EBV-CONTAINING NASOPHARYNGEAL CARCINOMAS
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DOI：
10.1002/ijc.2910420422
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发表时间：
1988-10-15
影响因子：
6.4
通讯作者：
TURSZ, T
中科院分区：
文献类型：
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作者：
BUSSON, P;GANEM, G;TURSZ, T
已获得3例可移植鼻咽癌（NPC）肿瘤，命名为C15、C17和C18。来源于原发性鼻咽癌肿瘤的C15已在裸鼠中繁殖了30代。来源于转移性鼻咽癌组织的C17和C18已传代10次。每个病例中都有桥粒，证实了所有3个肿瘤的上皮起源。Epstein‐Barr病毒（EBV）基因组在C15、C18和C17肿瘤细胞中分别含有30、12和3个拷贝。采用经典的抗补体免疫荧光（ACIF）技术对eb病毒核抗原（EBNA）进行染色。C15细胞荧光强度强，C18细胞荧光强度中等，C17细胞荧光强度几乎检测不到。EA和VCA抗原未见表达。单细胞悬液流式细胞术分析显示，在所有3例肿瘤中都没有可检测到的CR2分子（B淋巴细胞上的EBV受体），并且在C15和C17细胞中存在HLA - II类抗原的组成性表达。在体外培养3天的C15和C17细胞上清中显示IL‐1活性。这些数据证实，MHC - II类分子的合成和IL - 1样活性的释放是鼻咽癌细胞的常见特征。这些特征可能与鼻咽癌原发肿瘤中发现的T细胞浸润有关。
Three transplantable nasopharyngeal carcinoma (NPC) tumors, designated C15, C17 and C18, have been obtained and characterized. C15, derived from a primary NPC tumor, has been propagated in nude mice for 30 passages. C17 and C18, derived from metastatic NPC tissue, have been passaged 10 times. Desmosomes, present in every case, provided confirmation of the epithelial origin of all 3 tumors. The Epstein‐Barr virus (EBV) genome is contained in C15, C18 and C17 tumor cells with 30, 12 and 3 copies, respectively. The Epstein‐ Barr virus nuclear antigen (EBNA) was stained by the classical anti‐complement immunofluorescence (ACIF) technique. Fluorescence intensity was strong in C15, moderate in C18, and hardly detectable in C17 cells. No expression of the EA and VCA antigens was detected. Flow cytometry analysis performed on monocellular suspensions showed the absence of detectable CR2 molecules (the EBV receptor on B lymphocytes) in all 3 tumors, and the constitutive expression of HLA class‐II antigens in C15 and C17 cells. IL‐1 activity was demonstrated in the supernatant of C15 and C17 cells cultivatedin vitrofor 3 days. These data confirm that the constitutive synthesis of MHC class‐II molecules and the release of IL‐1‐like activities are frequent features of NPC cells. These characteristics could be of importance in relation with the T‐cell infiltrate found in NPC primary tumors.