Enhanced production of exopolysaccharides by fed-batch culture of Ganoderma resinaceum DG-6556.
Enhanced production of exopolysaccharides by fed-batch culture of Ganoderma resinaceum DG-6556.
复制标题
DOI：
--
复制
发表时间：
2006-04
影响因子：
3
通讯作者：
Hyun Mi Kim;S. Paik;K. Ra;Kwang Bon Koo;J. Yun;J. Choi
中科院分区：
文献类型：
--
作者：
Hyun Mi Kim;S. Paik;K. Ra;Kwang Bon Koo;J. Yun;J. Choi
本研究的目的是优化一个新的真菌分离物，树脂灵芝深层培养条件，并提高生物活性菌丝体生物量和胞外多糖（EPS）的流加培养。在初始pH 6.0和温度31 ℃下，在含有10 g/l葡萄糖、8 g/l大豆蛋白胨和5 mM MnCl（2）的培养基中，分批培养中菌丝生长和EPS产量达到最大。在优化了分批培养的培养基和环境条件后，采用补料分批培养策略来提高菌丝体生物量和EPS的产量。从培养物滤液的乙醇沉淀物的顶部或底部级分获得分子量范围为53，000至5，257，000 g/mol的五种不同EPS。采用流加培养的方法对G. resinaceum导致菌丝体生物量和EPS的产量增加。当在第6天将50 g/l葡萄糖进料到初始10 g/l葡萄糖培养基中时，获得菌丝体生物量（42.2 g/l）和EPS（4.6 g/l）的最大浓度。这可能是值得尝试的其他蘑菇发酵工艺，以提高蘑菇多糖的生产，特别是那些具有工业潜力的。
The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM MnCl(2) at an initial pH 6.0 and temperature 31 degrees C. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential.