SMA-3 smad has specific and critical functions in DBL-1/SMA-6 TGFbeta-related signaling.
SMA-3 smad has specific and critical functions in DBL-1/SMA-6 TGFbeta-related signaling.
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DOI：
10.1006/dbio.2000.9713
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发表时间：
2000-07
影响因子：
2.7
通讯作者：
C. Savage‐Dunn;R. Tokarz;H. Wang;S. Cohen;C. Giannikas;R. W. Padgett
中科院分区：
文献类型：
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作者：
C. Savage‐Dunn;R. Tokarz;H. Wang;S. Cohen;C. Giannikas;R. W. Padgett
在线虫中，转化生长因子β信号转导通路控制着身体大小和雄性尾巴的形态发生。我们已经分析了SMA-3Smad基因的功能，Sma-3Smad基因是在这一途径中发挥作用的三个Smad基因之一。SMA-3的零突变至少与配体和I型受体基因DBL-1和SMA-6的零突变一样严重，这表明其他Smad在没有SMA-3的情况下不起作用。此外，SMA-3的零突变不会导致产卵缺陷或发育受阻的达尔幼虫阶段的调节，这表明与线虫的另一条TGFbeta信号通路没有重叠的功能。SMA-3基因在两性和雄性发育的各个阶段都有广泛的表达。已经确定了与8个不同严重程度的SMA-3等位基因相关的分子损伤。错义突变聚集在先前发现的对Smad功能重要的两个区域。
A TGFbeta signal transduction cascade controls body size and male tail morphogenesis in the nematode Caenorhabditis elegans. We have analyzed the function of the sma-3 Smad gene, one of three Smad genes that function in this pathway. Null mutations in sma-3 are at least as severe as null mutations in the ligand and type I receptor genes, dbl-1 and sma-6, indicating that the other Smads do not function in the absence of SMA-3. Furthermore, null mutations in sma-3 do not cause defects in egg laying or in regulation of the developmentally arrested dauer larva stage, indicating no overlapping function with another C. elegans TGFbeta signaling pathway. The sma-3 gene is widely expressed at all developmental stages in hermaphrodites and males. The molecular lesions associated with eight sma-3 alleles of varying severity have been determined. The missense mutations cluster in two previously identified regions important for Smad function.