GO, A GTP-BINDING PROTEIN - IMMUNOCHEMICAL AND IMMUNOHISTOCHEMICAL LOCALIZATION IN THE RAT
GO, A GTP-BINDING PROTEIN - IMMUNOCHEMICAL AND IMMUNOHISTOCHEMICAL LOCALIZATION IN THE RAT
复制标题
DOI：
10.1111/j.1471-4159.1988.tb10588.x
复制
发表时间：
1988-04-01
影响因子：
4.7
通讯作者：
KATO, K
中科院分区：
文献类型：
--
作者：
ASANO, T;SEMBA, R;KATO, K
用免疫组织化学和免疫组织化学方法研究了GTP结合蛋白GO在大鼠体内的组织和细胞分布。因为针对阿尔法病毒的特异性抗体。牛围棋亚基(Go.alpha.)与大鼠Go.Alpha交叉反应，建立了牛Go.Alpha的酶免疫分析方法。被用于测量Go.Alpha的组织浓度。在老鼠身上。加油，阿尔法。除血细胞外，在所有被检查的组织中都检测到。Go.Alpha的浓度。在CNS(.apprx)中最高。大脑分别为7.7和4.4nmol/g)，其次是脑垂体腺和坐骨神经。在其他外周组织中，Go.α的浓度相对较高。在膀胱、胃和肠道中观察到；然而，这些值是大脑中浓度的2%。加油，阿尔法。在肠道中主要分布在肌层。免疫组织化学研究显示，Go.Alpha。主要与神经成分有关，但与每个外周器官特有的细胞无关。加油，阿尔法。也存在于神经内分泌细胞的膜上，包括垂体前叶的腺细胞、肾上腺髓质的嗜铬细胞、胰腺的胰岛细胞和甲状腺的滤泡旁细胞。这些结果表明，GO仅定位于神经组织和神经内分泌细胞。
The tissue and cellular distribution of a GTP-binding protein, Go, was investigated in the rat by immunochemical and immunohistochemical methods. Because the specific antibody for the .alpha. subunit of bovine Go (Go.alpha.) cross-reacted with rat Go.alpha., and enzyme immunoassay method developed for bovine Go.alpha. was applied for measuring the tissue concentration of Go.alpha. in the rat. Go.alpha. was detected in all tissues examined except blood cells. The concentration of Go.alpha. was highest in the CNS (.apprx. 7.7 and 4.4 nmol/g in the cerebrum, respectively), followed by the pituitary gland and sciatic nerve. Among the other peripheral tissues, relatively high concentrations of Go.alpha. were observed in the urinary bladder, stomach and intestines; however, these values were > 2% of the concentration in the cerebrum. Go.alpha. in the intestine was located mostly in the muscle layer. Immunohistochemical study showed that Go.alpha. was associated mostly with the neural elements but not with cells particular to each peripheral organ. Go.alpha. was also present in the membranes of neuroendocrine cells, including glandular cells in the anterior lobe of the pituitary gland, chromaffin cells in the medulla of the adrenal gland, islets cells in the pancreas, and parafollicular cells in the thyroid. These results indicate that Go is localized exclusively in the nervous tissues and neuroendocrine cells.