Regional localization of aβ-galactosidase locus on human chromosome 22
Regional localization of aβ-galactosidase locus on human chromosome 22
复制标题
人类 22 号染色体上 aβ-半乳糖苷酶基因座的区域定位
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
A. Westerveld
中科院分区：
文献类型：
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作者：
J. Wit;H. Hoeksema;D. Halley;A. Hagemeijer;D. Bootsma;A. Westerveld
将具有 X/22 易位 [46, XX, t(X; 22)(q23; q13)] 的人白细胞与中国仓鼠细胞融合。对分离的杂交体进行了人类染色体和 21 种酶标记的分析。开发了一种用于研究人-中国仓鼠杂交细胞中β-半乳糖苷酶同工酶的电泳技术。免疫学研究表明，在这些杂交种中研究的β-半乳糖苷酶标记物确实含有人类来源的免疫决定簇。此外，结果证明β-半乳糖苷酶基因座位于 22 号染色体上，远离 q13 断点。
Human white blood cells with an X/22 translocation [46, XX, t(X; 22)(q23; q13)] were fused with Chinese hamster cells. The isolated hybrids were analyzed for human chromosomes and 21 enzyme markers. An electrophoretic technique for studying the β-galactosidase isoenzymes in man-Chinese hamster hybrid cells was developed. Immunological studies showed that the β-galactosidase marker studied in these hybrids did contain immunological determinants of human origin. Furthermore the results provided evidence that a locus for β-galactosidase is situated on chromosome 22 distal to the breakpoint in q13.