Anti-tumor effect of Scutellaria barbata D. Don extracts on ovarian cancer and its phytochemicals characterisation
Anti-tumor effect of Scutellaria barbata D. Don extracts on ovarian cancer and its phytochemicals characterisation
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半枝莲提取物对卵巢癌的抗肿瘤作用及其植物化学成分表征
DOI：
10.1016/j.jep.2017.05.032
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发表时间：
2017-07-12
影响因子：
5.4
通讯作者：
Li, Weiling
中科院分区：
文献类型：
--
作者：
Zhang, Lin;Ren, Baoyin;Li, Weiling
民族药理学相关性：半枝莲D. Don是中国广泛使用的草药。它具有多种药用特性，包括抗氧化，抗炎和抗癌作用。本研究的目的是探讨紫藤是否能作为一种优良的药用植物。结果表明，唐宁对卵巢癌细胞的生长有明显的抑制作用，其作用机制有待于进一步研究。MTT法检测Don对卵巢癌A2780细胞活力的影响。通过DAPI染色和Annexin V-FITC染色分析细胞凋亡，通过细胞形态学观察来测量细胞凋亡。结果表明，半枝莲对卵巢癌细胞的迁移有明显的抑制作用。通过伤口愈合和transwell小室测定Don。观察了半枝莲对人卵巢癌细胞caspase 3/9、Bcl-2和MMP-2/9蛋白表达的影响。通过蛋白质印迹分析对Don进行评估。结果：采用HPLC-Q-TOF-MS对半枝莲粗提物进行了体外抗肿瘤活性研究。通过MTT法、DAPI染色法、伤口愈合实验、transwell迁移实验和western blotting分析，观察Don对卵巢癌A2780细胞的作用。本研究表明，紫茎泽兰D. Don通过下调Bcl-2蛋白表达和上调Caspase 3/9蛋白表达，降低A2780细胞的存活率，诱导细胞凋亡。此外，半枝莲D对A2780细胞的迁移有明显抑制作用。Don及其机制可能与MMP-2/9降低有关。从半枝莲中分离得到主要成分。Don被鉴定为13种黄酮类化合物。采用HPLC-Q-TOF-MS法对半枝莲的化学成分进行了分析。Don指出，半枝莲中含有14种黄酮类化合物，这些化合物可能与半枝莲的抗癌活性有关。结论：紫茎泽兰D. barbata D. Don可通过线粒体途径抑制A2780细胞增殖并诱导其凋亡。此外，半枝莲D. Don对卵巢癌细胞迁移的抑制作用与MMP-2/9表达下调有关。这些发现对卵巢癌的治疗有一定的指导意义。
Ethnopharmacological relevance: Scutellaria barbata D. Don is a widely used medicinal herb in China. It possess various medicinal properties including antioxidative, anti-inflammatory and anti-cancer effects. The aim of this study was to explore whether Scutellaria barbata D. Don could inhibit the growth of ovarian cancer cells in vitro and further investigate the underlying mechanisms.Materials and methods: Effects of Scutellaria barbata D. Don on the viability of ovarian cancer A2780 cells were measured by MTT assay. Apoptosis was measured by cell morphologic observation through DAPI staining and Annexin V-FITC staining assay for apoptosis analysis. The migration of ovarian cancer cells which exposed to Scutellaria barbata D. Don were measured by wound healing and transwell chamber assays. The protein levels of caspase 3/9, Bcl-2 and MMP-2/9 in human ovarian cancer cells treated with Scutellaria barbata D. Don were assessed by western blotting analysis. The potential bioactive compounds were characterized by HPLC-Q-TOF-MS.Results: The present study was to investigate the anticancer effects of crude extracts from Scutellaria barbata D. Don on ovarian cancer A2780 cells by MTT, DAPI staining, wound healing assay, transwell migration assay and western blotting analysis. Our study showed that Scutellaria barbata D. Don reduced the viability of A2780 cells and induced apoptosis by down-regulated Bcl-2 protein and increased Caspase 3/9 proteins. Furthermore, migration of A2780 cells were significantly inhibited by Scutellaria barbata D. Don and the underlying mechanism may be related to the decrease of MMP-2/9. The main constituents from Scutellaria barbata D. Don were identified to be thirteen flavonoids. A HPLC-Q-TOF-MS analysis of Scutellaria barbata D. Don indicated the presence of 14 flavonoids compounds, which may contribute to the anticancer activity of the Scutellaria barbata D. Don.Conclusions: Scutellaria barbata D. Don could inhibit proliferation and induce apoptosis in A2780 cells through mitochondrial pathway. Moreover, the inhibitory effect of Scutellaria barbata D. Don on the migration of ovarian cancer cells was associated with the down-regulation of MMP-2/9 expression. These findings could shed a light on the therapy of ovarian cancer.