ENHANCED MOTILITY IN NIH-3T3 FIBROBLASTS THAT OVEREXPRESS GELSOLIN
ENHANCED MOTILITY IN NIH-3T3 FIBROBLASTS THAT OVEREXPRESS GELSOLIN
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DOI：
10.1126/science.1848726
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发表时间：
1991-03-08
期刊：
影响因子：
56.9
通讯作者：
KWIATKOWSKI, DJ
中科院分区：
文献类型：
--
作者：
CUNNINGHAM, CC;STOSSEL, TP;KWIATKOWSKI, DJ
通过基因转染将培养的小鼠成纤维细胞中肌动蛋白结合蛋白凝溶胶蛋白的含量增加高达125%，成比例地提高了细胞通过多孔过滤器向血清梯度迁移的速率，并关闭了在组织培养皿中汇合单层细胞上形成的伤口。 这些结果提供了直接的证据，凝溶胶蛋白，促进肌动蛋白的组装和拆卸在体外，是一个重要的元素，在成纤维细胞运动，并表明，细胞内机器的操作可以增加细胞运动。
Increasing the content of the actin-binding protein gelsolin in cultured mouse fibroblasts by up to 125 percent by gene transfection proportionally enhanced the rate at which the cells migrated through porous filters toward a gradient of serum and closed a wound made on a confluent monolayer of cells in a tissue culture dish. These results provide direct evidence that gelsolin, which promotes both actin assembly and disassembly in vitro, is an important element in fibroblast locomotion and demonstrate that the manipulation of intracellular machinery can increase cell motility.