A late-acting quality control process for mature eukaryotic rRNAs
A late-acting quality control process for mature eukaryotic rRNAs
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DOI：
10.1016/j.molcel.2006.10.008
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发表时间：
2006-11-17
期刊：
影响因子：
16
通讯作者：
Moore, Melissa J.
中科院分区：
文献类型：
--
作者：
LaRiviere, Frederick J.;Cole, Sarah E.;Moore, Melissa J.
核糖体生物发生是一个多方面的过程，涉及许多反式作用因子介导的许多化学反应，RNA构象变化和RNA-蛋白质协会。鉴于这种高度的复杂性，严格的质量控制对于确保最终产品的结构和功能完整性可能至关重要。我们证明，核糖体RNA（rRNA）含有个别点突变，无论是在25 S肽基转移酶中心或18 S解码位点，对核糖体功能产生不利影响强烈下调酿酒酵母。这种下调通过降低完全组装的核糖体和核糖体亚基中所含的成熟rRNA的稳定性而发生。因此，真核生物具有一种质量控制机制，即非功能性rRNA衰变（NRD），能够检测和消除成熟核糖体的rRNA组分。
Ribosome biogenesis is a multifaceted process involving a host of trans-acting factors mediating numerous chemical reactions, RNA conformational changes, and RNA-protein associations. Given this high degree of complexity, tight quality control is likely crucial to ensure structural and functional integrity of the end products. We demonstrate that ribosomal RNAs (rRNAs) containing individual point mutations, in either the 25S peptidyl transferase center or 18S decoding site, that adversely affect ribosome function are strongly downregulated in Saccharomyces cerevisiae. This downregulation occurs via decreased stability of the mature rRNA contained in fully assembled ribosomes and ribosomal subunits. Thus, eukaryotes possess a quality-control mechanism, nonfunctional rRNA decay (NRD), capable of detecting and eliminating the rRNA component of mature ribosomes.