Alterations of lymphocyte populations during development in the spontaneously hypertensive rat
Alterations of lymphocyte populations during development in the spontaneously hypertensive rat
复制标题
自发性高血压大鼠发育过程中淋巴细胞群的变化
作者：
L. D. Fannon;R. Braylan;M. Phillips
目的：免疫系统异常与自发性高血压大鼠（SHR）高血压有关。我们研究的目的是研究高血压前期和SHR高血压发展阶段的不同淋巴细胞亚群。设计：SHR和Wistar-Kyoto （WKY）大鼠在以下时间点采集血样：2周和1、2、3和4个月。从全血中分离淋巴细胞。方法：采用单克隆抗体荧光标记以下淋巴细胞亚群；总T细胞，非辅助T细胞，辅助T细胞和B细胞。荧光活化细胞分选（FACS）分析用于量化所检查的不同亚群的百分比。结果：SHR 2周龄后T非辅助细胞群下降。这一发现在整个4个月的研究期间持续存在。在4个月的时间点，SHR的总T细胞百分比也有所下降。结论：这些结果表明免疫系统异常存在于SHR高血压前期和高血压发育阶段。这支持了一种假设，即免疫系统在不适应这种状态之前，参与了SHR中高血压的发展和维持。
Objective: Immune system abnormalities have been linked to hypertension in the spontaneously hypertensive rat (SHR). The goal of our study was to examine different lymphocyte subpopulations in the prehypertensive and developmental phases of hypertension in the SHR. Design: Blood samples were obtained from SHR and Wistar-Kyoto (WKY) rats at the following time-points: 2 weeks and 1, 2, 3 and 4 months. Lymphocytes were separated from the whole blood. Methods: Monoclonal antibodies were used to fluorescently label the following lymphocyte subpopulations; total T cells, T non-helper cells, T helper cells and B cells. Fluorescence-activated cell sorting (FACS) analysis was used to quantify the percentages of the different subpopulations examined. Results: The T non-helper cell population was depressed in SHR from 2 weeks of age. This finding persisted throughout the entire 4-month study period. At the 4-month time-point, the total T cell percentage was also depressed in the SHR. Conclusions: These results demonstrate that immune system abnormalities are present in the prehypertensive and developmental phases of hypertension in the SHR. This supports the hypothesis that the immune system is involved in the development and maintenance of hypertension in the SHR, preceding not adapting to this state.