Effect of Photobiomodulation on Transforming Growth Factor-β1, Platelet-Derived Growth Factor-BB, and Interleukin-8 Release in Palatal Wounds After Free Gingival Graft Harvesting: A Randomized Clinical Study.
Effect of Photobiomodulation on Transforming Growth Factor-β1, Platelet-Derived Growth Factor-BB, and Interleukin-8 Release in Palatal Wounds After Free Gingival Graft Harvesting: A Randomized Clinical Study.
复制标题
光生物调节对自由牙龈移植物收获后palat骨伤口中转化生长因子-β1，血小板衍生的生长因子Bb和白介素8释放的影响：一项随机临床研究。
DOI：
10.1089/pho.2016.4094
复制
发表时间：
2016-06
影响因子：
--
通讯作者：
Serdar MA
中科院分区：
文献类型：
--
作者：
Keskiner I;Lutfioğlu M;Aydogdu A;Saygun NI;Serdar MA
目的：本研究通过检测腭创面液（PWF）中转化生长因子(TGF)-β1、血小板衍生生长因子(PDGF)-BB和白细胞介素(IL)-8水平的变化，评价光生物调节（PBM）对游离龈移植（FGG）采收后供体腭区愈合的影响。材料与方法：选择30例患者，随机分为FGG采集后腭区PBM（激光组）和PBM假手术组（假手术组）。术后立即用掺钕钇铝石榴石（Nd:YAG）激光（1064 nm）照射试验点，此后每24 h照射一次，连续4天。于第7、12天收集PWF，采用酶联免疫吸附法（ELISA）检测PWF TGF-β1、PDGF-BB、IL-8水平。结果：两组患者PWF TGF-β1、PDGF-BB、IL-8水平在第12天均显著低于第7天。第7天，激光组PWF TGF-β1、PDGF-BB、IL-8水平显著高于假手术组（p < 0.05）。第12天，激光组PWF TGF-β1水平显著高于假手术组；然而，第12天各组间PDGF-BB和IL-8水平差异无统计学意义。结论：观察到PWF TGF-β1、PDGF-BB和IL-8水平的升高，表明PBM可能通过刺激特定介质的产生来加速伤口愈合。
Objective: This study evaluated the impact of photobiomodulation (PBM) on the healing of the donor palatal area following free gingival graft (FGG) harvesting by examining changes in transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF)-BB, and interleukin (IL)-8 levels in palatal wound fluid (PWF). Material and methods: Thirty patients were selected and randomly assigned to receive PBM (laser group) or PBM sham (sham group) in the palatine area after FGG harvesting. A neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (1064 nm) was applied to the test sites immediately after surgery and every 24 h thereafter for 4 days. PWF was collected on Days 7 and 12, and PWF TGF-β1, PDGF-BB, and IL-8 levels were analyzed by enzyme-linked immunosorbent assays (ELISA). Results: PWF TGF-β1, PDGF-BB, and IL-8 levels were significantly lower on Day 12 than on Day 7 for both groups. PWF TGF-β1, PDGF-BB, and IL-8 levels of the laser group were significantly higher than those of sham group on Day 7 (p < 0.05). PWF TGF-β1 levels were also significantly higher in laser group than in the sham group on Day 12; however, differences in PDGF-BB and IL-8 levels between groups on Day 12 were statistically nonsignificant. Conclusions: Observed increases in PWF TGF-β1, PDGF-BB, and IL-8 levels suggest that PBM may accelerate wound healing by stimulating production of selected mediators.