Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum
Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum
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DOI：
10.1016/j.bbamem.2015.03.005
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发表时间：
2015-06-01
影响因子：
3.4
通讯作者：
Sagami, Ikuko
中科院分区：
文献类型：
--
作者：
Ishijima, Sumio;Uda, Misaki;Sagami, Ikuko
镁（Mg 2+）在许多生理过程中起着关键作用。沙门氏菌中的Mg 2+转运系统已被充分记录，但大肠杆菌中的Mg 2+转运系统尚未完全阐明。研究了corA、mgtA、yhiD和corC基因缺失对大肠杆菌Mg ~（2+）转运的影响。杆菌我们获得了双重、三重和四重突变体的每一种组合。corA和mgtA双突变体需要向LuriaBertani（LB）培养基中添加10 mM Mg 2+以用于生长，而corA、mgtA和yhiD三突变体TM 2需要更高的Mg 2+浓度。四倍体突变体对Mg ~（2+）的需求量与TM 2相似。结果表明，CorA或MgtA是正常大肠杆菌所必需的。大肠杆菌LB培养基中的生长以及在高Mg ~（2+）生长条件下YhiD在Mg ~（2+）转运中的作用。杆菌拟南芥Mg ~（2+）转运蛋白AtMRS 2 -10和AtMRS 2 -11在TM 2细胞中异源表达。表达AtMRS 2 -10和AtMRS 2 -11的TM 2细胞可以分别在补充有1 mM Mg 2+和不补充Mg 2+的LB培养基中生长，并且细胞生长被2 mM AlCl 3抑制。结果表明，表达AtMRS 2 -10和AtMRS 2 -11的TM 2的生长反映了AtMRS 2对Mg ~（2+）和Al ~（2+）的功能。急诊coli TM 2细胞用于拟南芥MRS 2蛋白的功能分析。(C)2015 Elsevier B. V.版权所有。
Magnesium (Mg2+) plays a critical role in many physiological processes. Mg2+ transport systems in Salmonella have been well documented, but those in Escherichia coil have not been fully elucidated. We examined the effects of corA,mgtA, yhiD and corC gene deletion on Mg2+ transport in E. coli. We obtained every combination of double, triple and quadruple mutants. The corA and mgtA double mutant required addition of 10 mM Mg2+ to LuriaBertani (LB) medium for growth, and the corA, mgtA and yhiD triple mutant TM2 required a higher Mg2+ concentration. The Mg2+ requirement of the quadruple mutant was similar to that of TM2. The results demonstrated that either CorA or MgtA is necessary for normal E. coli growth in LB medium and that YhiD plays a role in Mg2+ transport under high Mg2+ growth conditions in E. coli. The Arabidopsis Mg2+ transporters, AtMRS2-10 and AtMRS2-11, were heterologously expressed in TM2 cells. TM2 cells expressing AtMRS2-10 and AtMRS2-11 could grow in LB medium that had been supplemented with 1 mM Mg2+ and without Mg2+ supplementation, respectively, and cell growth was inhibited by 2 mM AlCl3. The results indicated that the growth of TM2 expressing AtMRS2-10 and AtMRS2-11 reflected these AtMRS2 function for Mg2+ and aluminum. The E. coli TM2 cells are useful for functional analysis of Arabidopsis MRS2 proteins. (C) 2015 Elsevier B.V. All rights reserved.