Comparison of brain urocortin-3 and corticotrophin-releasing factor for physiological responses in chicks.
Comparison of brain urocortin-3 and corticotrophin-releasing factor for physiological responses in chicks.
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脑尿皮质素 3 和促肾上腺皮质激素释放因子对雏鸡生理反应的比较。
DOI：
10.1016/j.physbeh.2013.11.006
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Ogino;M.;Okumura;A.;Khan;M. S.;Cline;M. A.;Tachibana;T.
促肾上腺皮质激素释放激素(CRH)在应激反应中起重要作用，并通过其受体CRH受体-1(CRH-R1)和CRH受体-2(CRH-R2)在哺乳动物中发挥生理作用，与CRH-R1具有高亲和力。尿皮质激素-3(urocortin-3，UCN-3)是哺乳动物CRH-R2的内源性配体，属于CRH家族多肽。UCN-3和CRH-R2在哺乳动物中的生理作用已被研究，但它们在鸡(Gallus Gallus)中的作用仍需阐明。比较CRH和UCN-3对雏鸡的生理反应的研究很少。因此，本研究旨在研究侧脑室注射UCN-3对雏鸡摄食量、直肠温度、作物排空率和行为的影响，并与CRH注射的生理反应进行比较。侧脑室注射20和80nmolUCN-3可减少摄食量，提高直肠温度，降低作物排空率，其结果与CRH相似。注射UCN-3和CRH均增加自发活动，但行为模式不同：CRH增加发声次数，而UCN-3增加跳跃、扇翼和抓挠行为次数。这些结果表明，UCN-3通过CRH-R2调节雏鸡的摄食量、体温和胃排空，这些作用与CRH诱导的作用相似。
Corticotrophin-releasing hormone (CRH) plays an important role in response to stress, and exerts a physiological effect via its receptor, CRH receptor type-1 (CRH-R1) and CRH receptor type-2 (CRH-R2) with high affinity to CRH-R1 in mammals. Urocortin-3 (UCN-3), a CRH family peptide, is an endogenous ligand for CRH-R2 in mammals. The physiological roles of UCN-3 and CRH-R2 have been investigated in mammals, although their roles still need to be clarified in chicks (Gallus gallus). Few studies have been performed comparing the physiological responses of CRH and UCN-3 in chicks. Therefore the present study was conducted to investigate the effect of intracerebroventricular (ICV) injection of UCN-3 on food intake, rectal temperature, crop-emptying rate and behaviors in chicks, and to compare these physiological responses with the effects resulting from CRH injection. The ICV injection of 20 and 80 pmol UCN-3 decreased food intake, increased rectal temperature and decreased crop-emptying rate and the results were similar to those achieved with CRH. The injection of both UCN-3 and CRH increased spontaneous activity but the behavioral patterns were different: CRH increased the number of vocalizations while UCN-3 increased the number of jumps, wing-flaps and scratching behaviors. These results suggest that UCN-3 regulates food intake, body temperature, and gastric emptying via the CRH-R2 in the brain of chicks, and these effects were similar to those induced by CRH.